Evidence map›Paper›PMID 32238906›Full record

ArticleLaboratory investigation; a journal of technical methods and pathology2020

Increased talin-vinculin spatial proximities in livers in response to spotted fever group rickettsial and Ebola virus infections.

Yakun Liu, Jie Xiao, Ben Zhang, Thomas R Shelite, Zhengchen Su, Qing Chang, Barbara Judy, Xiang Li, Aleksandra Drelich, Jiani Bei and 10 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Laboratory investigation; a journal of technical methods and pathology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.4field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Talin‑1 interaction network in cellular mechanotransduction (Review).International journal of molecular medicine · 2022
    Review
  2. Article
  3. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors at 4 institutions in 3 countries.

Yakun Liu *Department of Pathology, University of Texas Medical Branch, Galveston, TX, 77555, USA.
Jie Xiao *Department of Pathology, University of Texas Medical Branch, Galveston, TX, 77555, USA.
Ben ZhangDepartment of Pathology, University of Texas Medical Branch, Galveston, TX, 77555, USA.
Thomas R SheliteDepartment of Internal Medicine, Infectious Diseases, University of Texas Medical Branch, Galveston, TX, 77555, USA.
Zhengchen SuDepartment of Pathology, University of Texas Medical Branch, Galveston, TX, 77555, USA.
Qing ChangDepartment of Pathology, University of Texas Medical Branch, Galveston, TX, 77555, USA.
Barbara JudyDepartment of Pathology, University of Texas Medical Branch, Galveston, TX, 77555, USA.
Xiang LiDepartment of Pathology, University of Texas Medical Branch, Galveston, TX, 77555, USA.
Aleksandra DrelichDepartment of Pathology, University of Texas Medical Branch, Galveston, TX, 77555, USA.
Jiani BeiDepartment of Pathology, University of Texas Medical Branch, Galveston, TX, 77555, USA.
Yixuan ZhouDepartment of Pathology, University of Texas Medical Branch, Galveston, TX, 77555, USA.
Junying ZhengDepartment of Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, TX, 77555, USA.
Yang JinDivision of Pulmonary and Critical Care Medicine, Department of Medicine, Boston University Medical Campus, Boston, MA, USA.
Shannan L RossiDepartment of Pathology, University of Texas Medical Branch, Galveston, TX, 77555, USA.
Shao-Jun TangDepartment of Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, TX, 77555, USA.
Maki WakamiyaDepartment of Neurology, University of Texas Medical Branch, Galveston, TX, 77555, USA.
Tais SaitoDepartment of Pathology, University of Texas Medical Branch, Galveston, TX, 77555, USA.
Thomas KsiazekDepartment of Pathology, University of Texas Medical Branch, Galveston, TX, 77555, USA.
Bhupendra KaphaliaDepartment of Pathology, University of Texas Medical Branch, Galveston, TX, 77555, USA.
Bin GongDepartment of Pathology, University of Texas Medical Branch, Galveston, TX, 77555, USA. bigong@utmb.edu.
The University of Texas Medical Branch at Galveston · USBoston University · USChanghai Hospital · CNTunghai University · TW

Funding

Wnt signaling in glial activation during HIV-associated chronic pain pathogenesisR01NS079166 · NINDS · UNIVERSITY OF TEXAS MED BR GALVESTON · PI TANG, SHAO-JUN · 2012 to 2021
$5.5M
Cooperative mechanisms of HIV and opiods in pain pathogenisisR01DA050530 · NIDA · UNIVERSITY OF TEXAS MED BR GALVESTON · PI CHUNG, JIN M, TANG, SHAO-JUN · 2019 to 2023
$3.2M
Antiretroviral Therapy and Neuroinflammation in the CNSR01NS095747 · NINDS · UNIVERSITY OF TEXAS MED BR GALVESTON · PI CHUNG, JIN M · 2016 to 2020
$2.6M
LncRNA regulates lung inflammationR01HL142758 · NHLBI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI JIN, YANG · 2019 to 2022
$2.0M
Epac1 Plays a Critical Role in Bacterial Adhesion during RickettsiosesR01AI121012 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI GONG, BIN · 2016 to 2020
$1.9M
Interplay of HIV-1 gp120 and opioids in astrocyte activationR01DA036165 · NIDA · UNIVERSITY OF TEXAS MED BR GALVESTON · PI TANG, SHAO-JUN · 2013 to 2017
$1.9M
Mechanistic insights of inflammation and organ failure after trauma or critical illnessR01GM127596 · NIGMS · BOSTON UNIVERSITY MEDICAL CAMPUS · PI JIN, YANG · 2018 to 2021
$1.3M
Targeting the Host Adenosine A2A Receptor to Protect Against Fatal Rickettsiosis Using an Approved Parkinson's Disease DrugR21AI137785 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI GONG, BIN · 2018 to 2019
$440k
The role of IL-1 family of cytokines in tick-transmitted ehrlichiosisR21AI144328 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI GONG, BIN · 2019 to 2020
$435k
Inflammasome activation and ehrlichial dissemination in tick vs needle transmissionR03AI142406 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI GONG, BIN · 2019 to 2020
$158k
NHLBI NIH HHS R01 HL142758NIAID NIH HHS R01 AI121012NIAID NIH HHS R03 AI142406NIAID NIH HHS R21 AI137785NIAID NIH HHS R21 AI144328NIDA NIH HHS R01 DA036165NIDA NIH HHS R01 DA050530NIGMS NIH HHS R01 GM127596NINDS NIH HHS R01 NS079166NINDS NIH HHS R01 NS095747
6 · The paper itself

Abstract

Talin and vinculin, both actin-cytoskeleton-related proteins, have been documented to participate in establishing bacterial infections, respectively, as the adapter protein to mediate cytoskeleton-driven dynamics of the plasma membrane. However, little is known regarding the potential role of the talin-vinculin complex during spotted fever group rickettsial and Ebola virus infections, two dreadful infectious diseases in humans. Many functional properties of proteins are determined by their participation in protein-protein complexes, in a temporal and/or spatial manner. To resolve the limitation of application in using mouse primary antibodies on archival, multiple formalin-fixed mouse tissue samples, which were collected from experiments requiring high biocontainment, we developed a practical strategic proximity ligation assay (PLA) capable of employing one primary antibody raised in mouse to probe talin-vinculin spatial proximal complex in mouse tissue. We observed an increase of talin-vinculin spatial proximities in the livers of spotted fever Rickettsia australis or Ebola virus-infected mice when compared with mock mice. Furthermore, using EPAC1-knockout mice, we found that deletion of EPAC1 could suppress the formation of spatial proximal complex of talin-vinculin in rickettsial infections. In addition, we observed increased colocalization between spatial proximity of talin-vinculin and filamentous actin-specific phalloidin staining in single survival mouse from an ordinarily lethal dose of rickettsial or Ebola virus infection. These findings may help to delineate a fresh insight into the mechanisms underlying liver specific pathogenesis during infection with spotted fever rickettsia or Ebola virus in the mouse model.

Indexed as

Actin CytoskeletonAnimalsCell MembraneCells, CulturedGuanine Nucleotide Exchange FactorsHemorrhagic Fever, EbolaHumansLiverMice, KnockoutProtein BindingRickettsiaSpotted Fever Group RickettsiosisTalinVinculinEpac protein, mouseGuanine Nucleotide Exchange FactorsTalinVinculin

Identifiers

PMID32238906
PMCPMC7111589
OpenAlexW3014212914

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.