Evidence map›Paper›PMID 32236842›Full record

ArticleMolecular biotechnology2020

Biochemical Characterization of VapC46 Toxin from Mycobacterium tuberculosis.

Madhurima Roy, Madhuparna Bose, Kamakshi Bankoti, Anirban Kundu, Santanu Dhara, Amit Kumar Das

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.1field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 12 citations in OpenAlex.

  1. Protein-Mediated Virulence in Mycobacterium tuberculosis.Advances in experimental medicine and biology · 2026
    Review
  2. Article
  3. Article
  4. Impact of Genomic Deletion RD16 on the Expression of theCurrent issues in molecular biology · 2023
    Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Madhurima RoyDepartment of Biotechnology, Indian Institute of Technology, Kharagpur, India.
Madhuparna BoseDepartment of Biotechnology, Indian Institute of Technology, Kharagpur, India.
Kamakshi BankotiSchool of Medical Science and Technology, Indian Institute of Technology, Kharagpur, India.
Anirban KunduDepartment of Biotechnology, Indian Institute of Technology, Kharagpur, India.
Santanu DharaSchool of Medical Science and Technology, Indian Institute of Technology, Kharagpur, India.
Amit Kumar DasDepartment of Biotechnology, Indian Institute of Technology, Kharagpur, India. amitk@bt.iitkgp.ac.in.ORCID http://orcid.org/0000-0001-9703-1898
Indian Institute of Technology Kharagpur · IN

Funding

Indian Institute of Technology Kharagpur IIT/SRIC/BIO/LDO/2014-15/33
6 · The paper itself

Abstract

Emergence of multidrug resistant strains and extremely drug resistant strains of Mycobacterium tuberculosis is due to its ability to form persister cells. The formation of persister cells is assumed to be triggered due to the presence of large number of toxin-antitoxin (TA) systems in its genome. Mtb genome encodes 47 VapBC TA systems. In this work, we aim to biochemically characterize VapC46 toxin of the VapBC46 TA operon from Mycobacterium tuberculosis. Heterologous expression of VapC46 in E. coli is shown to exhibit bacteriostasis and toxicity alters the surface morphology of the E. coli cells. VapC46 is shown to possess ribonuclease activity in a magnesium-dependent manner. Using FRET and pull down assay, VapC46 is shown to interact with VapB46 antitoxin. A model of VapC46 is shown to resemble PIN domain family of proteins and reveals the putative active site required for its ribonuclease activity.

Indexed as

Bacterial ProteinsBacterial ToxinsGenome, BacterialMycobacterium tuberculosisRibonucleasesToxin-Antitoxin SystemsBacterial ProteinsBacterial ToxinsRibonucleasesFRETMycobacterium tuberculosisPIN domainRibonucleaseToxin–antitoxin systemVapBC46

Identifiers

PMID32236842
OpenAlexW3014630436

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.