Evidence map›Paper›PMID 32236665›Full record

ArticlePediatric surgery international2020

Altered expression of AKT1 and P38A in the colons of patients with Hirschsprung's disease.

Lihua Wu, Ping Xiao, Qi Li, Zhen Zhang, Hui Wang, Qian Jiang, Long Li

Abstract read
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In one paragraph

Article in Pediatric surgery international, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Lihua WuDepartment of Medical Genetics, Capital Institute of Pediatrics, Beijing, 100020, China.
Ping XiaoDepartment of Pathology, Capital Institute of Pediatrics Affiliated Children's Hospital, Beijing, 100020, China.
Qi LiDepartment of General Surgery, Capital Institute of Pediatrics Affiliated Children's Hospital, No. 2 Yabao Rd., Chaoyang District, Beijing, 100020, China.
Zhen ZhangDepartment of General Surgery, Capital Institute of Pediatrics Affiliated Children's Hospital, No. 2 Yabao Rd., Chaoyang District, Beijing, 100020, China.
Hui WangDepartment of Medical Genetics, Capital Institute of Pediatrics, Beijing, 100020, China.
Qian JiangDepartment of Medical Genetics, Capital Institute of Pediatrics, Beijing, 100020, China.
Long LiDepartment of General Surgery, Capital Institute of Pediatrics Affiliated Children's Hospital, No. 2 Yabao Rd., Chaoyang District, Beijing, 100020, China. lilong23@126.com.
Capital Institute of Pediatrics · CNChildren's Hospital of Capital Institute of Pediatrics · CN

Funding

National Natural Science Foundation of China 81771620
6 · The paper itself

Abstract

purposeHirschsprung's disease (HSCR) is a functional obstruction of the gastrointestinal tract due to the congenital absence of enteric ganglion cells. The proto-oncogene RET is one of the primary genes implicated in the aetiology of HSCR. We designed this study to investigate the expression of 10 RET regulatory network genes in the colons of patients with HSCR.

methodsHSCR tissue specimens (n = 28) were collected at the time of pull-through surgery. qPCR analysis was applied to compare the expression levels of 10 genes in the RET regulatory network. Western blot analysis was performed to quantify the protein expression. Immunohistochemistry was performed to determine the localization of AKT1 and P38A in HSCR colon tissue.

resultsAKT1 (p = 0.015) and P38A (p = 0.039) were both significantly downregulated in the aganglionic segment compared to those in the ganglionic segment in HSCR patients (n = 28). Western blot analysis revealed the decreasing protein expression of AKT1 and P38A in the aganglionic segment compared to ganglionic segment and control colon tissues (p < 0.05). Immunohistochemistry staining revealed that both AKT1 and P38A were localized in the colonic mucosa and were significantly decreased in the aganglionic segment.

conclusionTo our knowledge, we report for the first time the expression of RET regulatory network genes in the colons of patients with HSCR. The markedly decreased expression of AKT1 and P38A suggested a possible role in HSCR pathogenesis.

Indexed as

Gene Expression RegulationBlotting, WesternDown-RegulationFemaleHirschsprung DiseaseHumansImmunohistochemistryInfantIntestinal MucosaMaleMitogen-Activated Protein Kinase 14Proto-Oncogene MasProto-Oncogene Proteins c-aktRNAAKT1 protein, humanMAS1 protein, humanMitogen-Activated Protein Kinase 14Proto-Oncogene MasProto-Oncogene Proteins c-aktRNAAKT1Gene regulatory networkHirschsprung’s diseaseP38A

Identifiers

PMID32236665
OpenAlexW3014339096

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.