Evidence map›Paper›PMID 32235304›Full record

ArticleBiomolecules2020

A High-Throughput Single-Clone Phage Fluorescence Microwell Immunoassay and Laser-Driven Clonal Retrieval System.

Seohee Chang, Soohyun Kim, Jerome Han, Suji Ha, Hyunho Lee, Seo Woo Song, Daewon Lee, Sunghoon Kwon, Junho Chung, Junhoi Kim

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomolecules, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Seohee ChangDepartment of Electrical and Computer Engineering, Seoul National University, Seoul 08826, Korea.ORCID 0000-0003-0276-7013
Soohyun KimDepartment of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul National University, Seoul 03080, Korea.
Jerome HanDepartment of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul National University, Seoul 03080, Korea.
Suji HaDepartment of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul National University, Seoul 03080, Korea.
Hyunho LeeDepartment of Electrical and Computer Engineering, Seoul National University, Seoul 08826, Korea.
Seo Woo SongBio-Max Institute, Seoul National University, Seoul 08826, Korea.
Daewon LeeBK21+ Creative Research Engineer Development for IT, Seoul National University, Seoul 08826, Korea.ORCID 0000-0002-1268-2542
Sunghoon KwonDepartment of Electrical and Computer Engineering, Seoul National University, Seoul 08826, Korea.
Junho ChungDepartment of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul National University, Seoul 03080, Korea.
Junhoi KimCancer Research Institute, Seoul National University College of Medicine, Seoul National University, Seoul 03080, Korea.ORCID 0000-0002-8069-5935
Seoul National University · KR

Funding

Global Research Development Center Program through the NRF funded by the Ministry of Science and ICT (MSIT) 2015K1A4A3047345Korea Drug Development Fund KDDF-201904-19National Research Foundation of Korea (NRF) 2016M3A9B5941738National Research Foundation of Korea (NRF) 2017R1A6A3A04010692
6 · The paper itself

Abstract

Phage display is one of the most frequently used platform technologies utilized to screen and select therapeutic antibodies, and has contributed to the development of more than 10 therapeutic antibodies used in the clinic. Despite advantages like efficiency and low cost, it has intrinsic technical limitations, such as the asymmetrical amplification of the library after each round of biopanning, which is regarded as a reason for it yielding a very limited number of antigen binders. In this study, we developed a high-throughput single-clonal screening system comprised of fluorescence immunoassays and a laser-driven clonal DNA retrieval system using microchip technology. Using this system, from a single-chain variable fragment (scFv) library displayed on phages with a complexity of 5.21 × 10

Indexed as

FluorescenceLasersPeptide LibraryHumansImmunoassayPeptide Librarymicrochipphage displaysingle clone

Identifiers

PMID32235304
PMCPMC7226094
OpenAlexW3013256213

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.