ArticleAnimals : an open access journal from MDPI2020
Identification of Plasma Fatty Acids in Four Lipid Classes to Understand Energy Metabolism at Different Levels of Ketonemia in Dairy Cows Using Thin Layer Chromatography and Gas Chromatographic Techniques (TLC-GC).
Article in Animals : an open access journal from MDPI, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Field Evaluation of Liver Ultrasound Measurements and Biochemical and Metabolic Parameters During the Transition Period in Dairy Cows.Animals : an open access journal from MDPI · 2025Article
- Comprehensive Characterization of Serum Lipids of Dairy Cows: Effects of Negative Energy Balance on Lipid Remodelling.Metabolites · 2025Article
- Critical thresholds of BHBA and NEFA for subclinical ketosis and its prevalence prediction in Indian mithun (Bos frontalis) cows demonstrated by ROC analyses.Tropical animal health and production · 2025Article
- Application of lipidomics in the study of traditional Chinese medicine.Journal of pharmaceutical analysis · 2025Review
- Near-infrared spectroscopy analysis of blood plasma for predicting nonesterified fatty acid concentrations in dairy cows.JDS communications · 2024Article
- Metabolic Changes Associated with Different Levels of Energy Deficits in Mediterranean Buffaloes during the Early Lactation Stage: Type and Role of the Main Lipid Fractions Involved.Animals : an open access journal from MDPI · 2023Article
- Filling gaps in animal welfare assessment through metabolomics.Frontiers in veterinary science · 2023Review
- Association between Fatty Acid Composition in Hair and Energy Availability during Early Lactation in Simmental and German Holstein Cows.Metabolites · 2022Article
- Dietary and Animal Strategies to Reduce the Environmental Impact of Pastoral Dairy Systems Result in Altered Nutraceutical Profiles in Milk.Animals : an open access journal from MDPI · 2022Article
- Differences in the serum metabolome profile of dairy cows according to the BHB concentration revealed by proton nuclear magnetic resonance spectroscopy (Scientific reports · 2022Article
- Myostatin Knockout Regulates Bile Acid Metabolism by Promoting Bile Acid Synthesis in Cattle.Animals : an open access journal from MDPI · 2022Article
- Optimised Method for Short-Chain Fatty Acid Profiling of Bovine Milk and Serum.Molecules (Basel, Switzerland) · 2022Article
- Milk Fatty Acids Composition Changes According to β-Hydroxybutyrate Concentrations in Ewes during Early Lactation.Animals : an open access journal from MDPI · 2021Article
- Changes of Plasma Fatty Acids in Four Lipid Classes to Understand Energy Metabolism at Different Levels of Non-Esterified Fatty Acid (NEFA) in Dairy Cows.Animals : an open access journal from MDPI · 2020Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Excessive mobilization of adipose tissue in high milk producing dairy cows predisposes to metabolic diseases. The aim of this research was to identify the plasma fatty acids in four lipid classes as biomarkers for the diagnosis of hyperketonemia in bovines using thin layer chromatography and gas chromatographic techniques (TLC-GC). Sixty multiparous Holstein-Friesian dairy cows were enrolled in the study. Blood samples from the coccygeal vein were collected and β-hydroxybutyrate (BHB) was evaluated. All animals were divided into three groups on the basis of ketonemia: BHB < 0.50 mmol/L, 0.50 < BHB < 1.0 mmol/L, and BHB > 1.0 mmol/L. Plasma fatty acid concentrations were evaluated in four lipid classes: Free Fatty Acids (FFA), Triglycerides (TG), Cholesterol Esters (CE) And Phospholipids (PL). The concentration of fatty acids was analyzed using TLC-GC. The results showed the following significance in the lipid classes: 19 fatty acids were significant (
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