Evidence map›Paper›PMID 32234059›Full record

ArticleBMC research notes2020

Whole genome bisulfite sequencing of Medicago truncatula A17 wild type and lss mutants.

Nowlan H Freese, Elise L Schnabel, Julia A Frugoli

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC research notes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Nowlan H FreeseDepartment of Bioinformatics and Genomics, University of North Carolina at Charlotte, Kannapolis, NC, 28081, USA.
Elise L SchnabelDepartment of Genetics & Biochemistry, Clemson University, Clemson, SC, 29634, USA.
Julia A FrugoliDepartment of Genetics & Biochemistry, Clemson University, Clemson, SC, 29634, USA. jfrugol@clemson.edu.ORCID http://orcid.org/0000-0003-1642-5911
Clemson University · USUniversity of North Carolina at Charlotte · US

Funding

National Science Foundation 1523814National Science Foundation IOS 1444461
6 · The paper itself

Abstract

objectivesEarlier work in our lab identified a spontaneous mutant (likesunnsupernodulator-lss) in Medicago truncatula, resulting in increased nodulation. Molecular genetic evidence indicated the phenotype was due to an unknown lesion resulting in cis-silencing of the SUNN gene. Altered methylation of the promoter was suspected, but analysis of the SUNN promoter by bisulfite sequencing at the time of publication revealed no significant methylation differences between the SUNN promoter in wild type and lss plants. Using advances in methylome generation we compared the methylome of wild type and the lss mutant in the larger 810 kB area of the genome where lss maps. DATA DESCRIPTION: The data show the distribution of types of methylation across the entire genome between A17 wild type and lss mutants, the number of differentially methylated cytosines between genotypes, and the overall pattern of gene methylation between genotypes. We expect the wild type data will be especially useful as a reference for other investigations of methylation using M. truncatula.

Indexed as

DNA MethylationGene Expression Regulation, PlantGenome, PlantMedicago truncatulaSequence Analysis, DNASulfiteshydrogen sulfiteSulfitesAutoregulation of nodulationlssMedicago truncatulaMethylationNodulation

Identifiers

PMID32234059
PMCPMC7110649
OpenAlexW3014593408

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.