ArticleKorean journal of ophthalmology : KJO2020
Effect of Minoxidil on Trabecular Outflow via the Paracellular Pathway.
Article in Korean journal of ophthalmology : KJO, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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2 authors at 2 institutions in 1 country.
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Abstract
purposeTo investigate the pathway and effects of minoxidil on trabecular outflow in cultured human trabecular meshwork (TM) cells.
methodsAfter exposing primarily cultured TM cells to 0, 10, 50, or 100 μM minoxidil sulfate (MS), trabecular outflow was assessed by measuring TM cell monolayer permeability to carboxyfluorescein and transepithelial electrical resistance. To assess the pathway of permeability changes, caveolin-1, occludin, and claudin-5 levels were measured via western blot. Generation of reactive oxygen species (ROS) was measured using the dichlorofluorescein diacetate assay. To assess the involvement of nitric oxide (NO) in minoxidil-induced permeability increase, the degrees of endothelial nitric oxide synthase mRNA expression and NO production were measured with reverse transcription polymerase chain reaction and Griess assays, respectively. Permeability was also measured with co-exposure to 50 μM N-acetyl cysteine.
resultsMS significantly increased TM cell monolayer permeability (
conclusionsMinoxidil may preferentially increase trabecular permeability via a paracellular pathway by downregulation of tight junction proteins. This minoxidil-induced permeability through the TM may be mediated by generation of ROS.
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