ArticleEndocrine2020
Performance of a dual-component molecular assay in cytologically indeterminate thyroid nodules.
Article in Endocrine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 5 of them syntheses that pooled it.
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Who cites it
20 citing papers in PubMed, 5 syntheses or guidelines pooled it, 37 citations in OpenAlex.
- A systematic review of emerging RNA markers in thyroid fine needle aspiration cytology samples: advancements and challenges.Endocrine · 2025Pooled it
- 2023 European Thyroid Association Clinical Practice Guidelines for thyroid nodule management.European thyroid journal · 2023Guideline
- Thyroid Nodules with Indeterminate FNAC According to the Italian Classification System: Prevalence, Rate of Operation, and Impact on Risk of Malignancy. An Updated Systematic Review and Meta-analysis.Endocrine pathology · 2022Pooled it
- Pooled it
- The ultrasound risk stratification systems for thyroid nodule have been evaluated against papillary carcinoma. A meta-analysis.Reviews in endocrine & metabolic disorders · 2021Pooled it
- Design and analytical validation of Nexthyro 2.0, a custom next-generation sequencing panel integrating DNA mutation profiling and RNA fusion detection for routine thyroid pathology specimens.Virchows Archiv : an international journal of pathology · 2026Article
- Comparing the diagnostic accuracy of pre-operative genetic testing for thyroid cancer on fine needle aspiration cytology specimens: a systematic review and meta-analysis of diagnostic accuracy.Discover oncology · 2025Article
- Serum microRNA-146a-5p and microRNA-221-3p as potential clinical biomarkers for papillary thyroid carcinoma.Journal of endocrinological investigation · 2025Observational
- The diagnostic and prognostic role of miR-146b-5p in differentiated thyroid carcinomas.Frontiers in endocrinology · 2024Article
- The role of miR-139-5p in radioiodine-resistant thyroid cancer.Journal of endocrinological investigation · 2023Article
- Molecular testing in indeterminate thyroid nodules: an additional tool for clinical decision-making.Pathologica · 2023Review
- Article
- Establishment and maintenance of thyroid organoids from human cancer cells.STAR protocols · 2022Article
- Thyroid Nodule Characterization: How to Assess the Malignancy Risk. Update of the Literature.Diagnostics (Basel, Switzerland) · 2021Review
- Review
- Long-term disease recurrence in the adipose tissue and striated muscles of a minimally invasive papillary thyroid carcinoma.Endocrine · 2021Article
- Raman Spectroscopy Discloses Altered Molecular Profile in Thyroid Adenomas.Diagnostics (Basel, Switzerland) · 2020Article
- Contemporary Thyroid Nodule Evaluation and Management.The Journal of clinical endocrinology and metabolism · 2020Review
- Analytical validation of a novel targeted next-generation sequencing assay for mutation detection in thyroid nodule aspirates and tissue.Endocrine · 2020Article
- Loss of Function SETD2 Mutations in Poorly Differentiated Metastases from Two Hürthle Cell Carcinomas of the Thyroid.Cancers · 2020Article
Corrections and comments
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Authors and funding
15 authors at 4 institutions in 1 country.
Funding
Abstract
purposeDeciding whether patients with a cytologically indeterminate thyroid nodule should be referred for surgery or for active surveillance is an important challenge for clinicians. The aim of this study was to evaluate the performance of a novel dual-component molecular assay as an ancillary molecular method for resolving indeterminate thyroid nodule cytology.
methodsWe selected 156 thyroid nodules from those that had undergone fine-needle aspiration processed by liquid-based cytology and surgical resection between June 2016 and December 2017. The sample set included 63 nodules cytologically classified as indeterminate, and 93 other nodules randomly selected from those with non-diagnostic, benign, suspicious, or malignant cytology. Nucleic acids from each nodule were subjected to next-generation sequencing analysis for mutation detection in 23 genes and to digital polymerase chain reaction (PCR) evaluation for miR-146b-5p expression levels.
resultsUsed alone, mutation analysis in the indeterminate subset (cancer prevalence: 22.5%) displayed high sensitivity (89%) and NPV (96%). In contrast, the miR-146b-5p assay offered high specificity (93%) and PPV (93%). Combined use of both analyses improved panel performance by eliminating false-negative results.
conclusionsThese preliminary data suggest that a dual-component molecular test can increase the diagnostic accuracy of thyroid cytology alone by reducing the number of nodules that will be classified as indeterminate and increasing those that can be reliably classified as benign. If these findings are confirmed, this test can be considered for use in clinical practice and is expected to reduce diagnostic surgery and health care costs, and to improve patient quality of life.
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