ArticleNucleic acids research2020
A GoldenBraid cloning system for synthetic biology in social amoebae.
Article in Nucleic acids research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Who cites it
17 citing papers in PubMed.
- Eukaryotic domestication of a bacterial immune protein following horizontal transfer.bioRxiv : the preprint server for biology · 2026Article
- Unlocking CRISPR-Cas9 editing for widely diverse Dictyostelid species.Molecular systems biology · 2026Article
- Early cAMP signaling orchestrates single-cell synchronicity throughout Dictyostelium development.Communications biology · 2026Article
- Complementary cytoskeletal feedback loops control signal transduction excitability and cell polarity.Nature communications · 2025Article
- Development of "Tea Rice" by engineering catechin biosynthesis in rice endosperm.Plant biotechnology journal · 2025Article
- Advancements in Golden Gate Cloning: A Comprehensive Review.Methods in molecular biology (Clifton, N.J.) · 2025Review
- GoldenBraid2.0Synthetic biology (Oxford, England) · 2025Article
- Multi-color fluorescence live-cell imaging in Dictyostelium discoideum.Cell structure and function · 2024Article
- Going against the family: Perturbation of a greenbeard pathway leads to falsebeard cheating.iScience · 2024Article
- The greenbeard gene tgrB1 regulates altruism and cheating in Dictyostelium discoideum.Nature communications · 2024Article
- Collective signalling drives rapid jumping between cell states.Development (Cambridge, England) · 2023Article
- Golden Standard: a complete standard, portable, and interoperative MoClo tool for model and non-model proteobacteria.Nucleic acids research · 2023Article
- Synthetic Assembly DNA Cloning to Build Plasmids for Multiplexed Transgenic Selection, Counterselection or Any Other Genetic Strategies Using Drosophila melanogaster.Current protocols · 2023Article
- Article
- A User's Guide to Golden Gate Cloning Methods and Standards.ACS synthetic biology · 2022Review
- Transcriptional milestones inGenome research · 2021Article
- The GB4.0 Platform, an All-In-One Tool for CRISPR/Cas-Based Multiplex Genome Engineering in Plants.Frontiers in plant science · 2021Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elements. Dictyostelium amoebae represent an intriguing synthetic biological chassis with tractable applications in development, chemotaxis, bacteria-host interactions, and allorecognition. We present GoldenBraid as a synthetic biological framework for Dictyostelium, including a library of 250 DNA parts and assemblies and a proof-of-concept strain that illustrates cAMP-chemotaxis with four fluorescent reporters coded by one plasmid.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.