ArticleScience advances2020
Streptavidin/biotin: Tethering geometry defines unbinding mechanics.
Article in Science advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
44 citing papers in PubMed.
- Recent Advances in Conjugation Chemistry Expanding the Applications of the DNA Tetrahedron.Chemical biology & drug design · 2026Review
- Single-Molecule Conductance of Non-Redox Proteins: Mechanisms, Measurements, and Applications.Biomolecules · 2026Review
- DNA-Guided Robust Single-Protein Electronic Readout.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Single-molecule mechanostructural fingerprinting of nucleic acid conformations.Nucleic acids research · 2026Article
- UltrastrongScience advances · 2025Article
- Design and Assembly of a Cargo-Agnostic Hollow Two-Lidded DNA Origami Box.ACS applied bio materials · 2025Article
- A Universal Approach to Anchoring Chromophores onto Magnetic Scaffold for Achieving Easily Recyclable Heterogeneous Photocatalytic Systems.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Mesothelin-Mediated Paclitaxel Phase-Shifted Nanodelivery System for Molecular Ultrasound Imaging and Targeted Therapy Potential in Ovarian Cancer.Current drug delivery · 2025Article
- The strongest protein binder is surprisingly labile.Protein science : a publication of the Protein Society · 2024Article
- Engineering the Mechanical Stability of a Therapeutic Affibody/PD-L1 Complex by Anchor Point Selection.bioRxiv : the preprint server for biology · 2024Article
- Brain-targeted drug delivery - nanovesicles directed to specific brain cells by brain-targeting ligands.Journal of nanobiotechnology · 2024Review
- Super-resolution proximity labeling with enhanced direct identification of biotinylation sites.Communications biology · 2024Article
- Engineering an artificial catch bond using mechanical anisotropy.Nature communications · 2024Article
- Identification of Individual Target Molecules Using Antibody-Decorated DeepTipBiomimetics (Basel, Switzerland) · 2024Article
- Uncovering receptor-ligand interactions using a high-avidity CRISPR activation screening platform.Science advances · 2024Article
- Mapping Single-Molecule Protein Complexes in 3D with DNA Nanoswitch Calipers.Journal of the American Chemical Society · 2023Article
- Statistical Study of Low-Intensity Single-Molecule Recognition Events Using DeepTipBiomimetics (Basel, Switzerland) · 2023Article
- Sulindac selectively induces autophagic apoptosis of GABAergic neurons and alters motor behaviour in zebrafish.Nature communications · 2023Article
- Fostering discoveries in the era of exascale computing: How the next generation of supercomputers empowers computational and experimental biophysics alike.Biophysical journal · 2023Review
- Highly sensitive and label-free detection of biotin using a liquid crystal-based optofluidic biosensor.Biomedical optics express · 2023Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Macromolecules tend to respond to applied forces in many different ways. Chemistry at high shear forces can be intriguing, with relatively soft bonds becoming very stiff in specific force-loading geometries. Largely used in bionanotechnology, an important case is the streptavidin (SA)/biotin interaction. Although SA's four subunits have the same affinity, we find that the forces required to break the SA/biotin bond depend strongly on the attachment geometry. With AFM-based single-molecule force spectroscopy (SMFS), we measured unbinding forces of biotin from different SA subunits to range from 100 to more than 400 pN. Using a wide-sampling approach, we carried out hundreds of all-atom steered molecular dynamics (SMD) simulations for the entire system, including molecular linkers. Our strategy revealed the molecular mechanism that causes a fourfold difference in mechanical stability: Certain force-loading geometries induce conformational changes in SA's binding pocket lowering the energy barrier, which biotin has to overcome to escape the pocket.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.