ArticleeLife2020
Approaching boiling point stability of an alcohol dehydrogenase through computationally-guided enzyme engineering.
Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
19 citing papers in PubMed, 59 citations in OpenAlex.
- Combined computational, rational, and empirical design of boiling-resistant keratinase.Applied and environmental microbiology · 2026Article
- Enriching stabilizing mutations through automated analysis of molecular dynamics simulations using BoostMut.Protein science : a publication of the Protein Society · 2025Article
- Decoding Protein Stabilization: Impact on Aggregation, Solubility, and Unfolding Mechanisms.Journal of chemical information and modeling · 2025Article
- A Genetically Encoded Redox-Active Nicotinamide Amino Acid.Biochemistry · 2024Article
- Novel Insights into Enzymatic Thermostability: The "Short Board" Theory and Zero-Shot Hamiltonian Model.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Catalytic Stability ofInternational journal of molecular sciences · 2024Article
- Solvent concentration at 50% protein unfolding may reform enzyme stability ranking and process window identification.Nature communications · 2024Article
- Article
- Advancing Enzyme's Stability and Catalytic Efficiency through Synergy of Force-Field Calculations, Evolutionary Analysis, and Machine Learning.ACS catalysis · 2023Article
- Structure- and computational-aided engineering of an oxidase to produce isoeugenol from a lignin-derived compound.Nature communications · 2022Article
- Engineering functional thermostable proteins using ancestral sequence reconstruction.The Journal of biological chemistry · 2022Review
- Enhancing the Thermostability of Phytase to Boiling Point by Evolution-Guided Design.Applied and environmental microbiology · 2022Article
- Broadening the Scope of the Flavin-Tag Method by Improving Flavin Incorporation and Incorporating Flavin Analogs.Chembiochem : a European journal of chemical biology · 2022Article
- Tuning Enzyme Thermostability via Computationally Guided Covalent Stapling and Structural Basis of Enhanced Stabilization.Biochemistry · 2022Article
- Enhancing computational enzyme design by a maximum entropy strategy.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Stabilizing AqdC, a Pseudomonas Quinolone Signal-Cleaving Dioxygenase from Mycobacteria, by FRESCO-Based Protein Engineering.Chembiochem : a European journal of chemical biology · 2021Article
- Power of Biocatalysis for Organic Synthesis.ACS central science · 2021Review
- Using Mutability Landscapes To Guide Enzyme Thermostabilization.Chembiochem : a European journal of chemical biology · 2021Article
- Harnessing Conformational Plasticity to Generate Designer Enzymes.Journal of the American Chemical Society · 2020Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 5 countries.
Funding
Abstract
Enzyme instability is an important limitation for the investigation and application of enzymes. Therefore, methods to rapidly and effectively improve enzyme stability are highly appealing. In this study we applied a computational method (FRESCO) to guide the engineering of an alcohol dehydrogenase. Of the 177 selected mutations, 25 mutations brought about a significant increase in apparent melting temperature (Δ
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.