ArticleBMC cancer2020
Evaluation of cinnamon extract effects on clbB gene expression and biofilm formation in Escherichia coli strains isolated from colon cancer patients.
Article in BMC cancer, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- Stage-Specific carcinogenic mechanisms and intervention strategies for pksArchives of microbiology · 2026Review
- Essential Oils as Antimicrobial Agents Against WHO Priority Bacterial Pathogens: A Strategic Review of In Vitro Clinical Efficacy, Innovations and Research Gaps.Antibiotics (Basel, Switzerland) · 2025Review
- The interactions of natural compounds with Escherichia coli motility, attachment, communication systems, and mature biofilm.Archives of microbiology · 2025Review
- Essential Oils for Biofilm Control: Mechanisms, Synergies, and Translational Challenges in the Era of Antimicrobial Resistance.Antibiotics (Basel, Switzerland) · 2025Review
- Investigating the effects of vitamin D3 and 5-aminosalicylic acid (Mesalazine) on the expression of virulence determinants inGastroenterology and hepatology from bed to bench · 2025Article
- Article
- Cinnamaldehyde: Pharmacokinetics, anticancer properties and therapeutic potential (Review).Molecular medicine reports · 2024Review
- The role and mechanism of cinnamaldehyde in cancer.Journal of food and drug analysis · 2024Review
- Current understandings of colibactin regulation.Microbiology (Reading, England) · 2024Review
- Inhibitory effect of natural compounds on quorum sensing system inFrontiers in pharmacology · 2024Review
- D-Serine reduces the expression of the cytopathic genotoxin colibactin.Microbial cell (Graz, Austria) · 2023Article
- Role of adherent invasiveWorld journal of clinical cases · 2022Review
- Evaluation of Catechin Synergistic and Antibacterial Efficacy on Biofilm Formation andAntibiotics (Basel, Switzerland) · 2022Article
- Natural Compounds: A Hopeful Promise as an Antibiofilm Agent AgainstFrontiers in pharmacology · 2022Review
- Review
- Antibacterial Activity of T22, a Specific Peptidic Ligand of the Tumoral Marker CXCR4.Pharmaceutics · 2021Article
- Review
- Oligosaccharides increase the genotoxic effect of colibactin produced by pks+ Escherichia coli strains.BMC cancer · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundColon cancer is one of the most common malignancies and the fourth leading cause of cancer-related mortality in the world. Colibactin, which is synthesized by the pks genomic island of E. coli interfere with the eukaryotic cell cycle. Cinnamon has an antimicrobial effect and considered as a colon cancer-preventing agent. The aim of the study was to evaluate the effects of cinnamon extract and cinnamaldehyde on clbB gene expression and biofilm formation in clinical isolates of E. coli.
methodsThirty E. coli carrying pks gene were isolated from the colon cancer patients, inflammatory bowel disease and healthy subjects. Antibiotic susceptibility was evaluated by disk diffusion method and the minimum inhibitory concentration of cinnamon essential oil and cinnamaldehyde by microdilution broth method. In vitro biofilm formation of E.coli isolates was monitored using a microtiter plate method. The presence of clbB, clbA and clbQ genes in E.coli isolates were evaluated by PCR. The effect of cinnamaldehyde and cinnamon essential oil on clbB gene expression was evaluated by Real-Time PCR.
resultsThe highest antibiotic resistance was obtained with 94.4% for ticarcillin-clavulanic acid, azithromycin, amoxicillin, and amikacin. The MIC for all clinical isolates was 32 μl/ml of cinnamon essential oil and the MIC of cinnamaldehyde was between 0.00002 to 0.03 μl/ml. After exposure of isolates to cinnamon extract and cinnamaldehyde, 40 and 13.3% were weakly biofilm producers, respectively. The frequencies of clbB, clbA, and clbQ genes were 23.3, 23.3, and 26.7%, respectively. The expression of clbB gene in the presence of the Sub-MIC concentration of cinnamon essential oil and cinnamaldehyde was decreased in 8 isolates compared to untreated isolates (p-value < 0.05).
conclusionsThe antibacterial activity of cinnamaldehyde and cinnamon essential oil allows the use of these herbal compounds for treatment or supplements in infections caused by E. coli and in patients with suspected colorectal cancer.
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