ArticleJournal of proteome research2020
Optimization of Synovial Fluid Collection and Processing for NMR Metabolomics and LC-MS/MS Proteomics.
Article in Journal of proteome research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Enrichment and Proteomic Profiling of Stromal-Associated Extracellular Vesicle Subpopulations From Arthritic Synovial Fluid by Size-Exclusion Chromatography Coupled to CD90-Directed Immunocapture.Journal of extracellular vesicles · 2026Article
- A novel diagnostic technology rapidly detects the presence of infection and defines antimicrobial sensitivities in organisms typical for joint infection.Bone & joint research · 2026Article
- Evaluating Protein Enrichment Methods to Improve Biomarker Discovery in Equine Cerebrospinal Fluid.Veterinary medicine and science · 2026Article
- Systematic evaluation of pre-analytical variables on synovial fluid metabolomic profiles using GC-ToF-MS and UHPLC-MS.The Analyst · 2026Article
- Ozone therapy in femoral head necrosis: a perspective on biomarker-guided treatment rationalization and mechanistic exploration.Frontiers in medicine · 2026Article
- Metabolomic and proteomic stratification of equine osteoarthritis.Equine veterinary journal · 2025Article
- How Does Tendon Region, Donor, and the Presence of Disease Affect Protein Composition of the Achilles Tendon?Clinical orthopaedics and related research · 2025Article
- Article
- C1QA and COMP: plasma-based biomarkers for early diagnosis of pancreatic neuroendocrine tumors.Scientific reports · 2023Article
- Low-Abundance Protein Enrichment for Medical Applications: The Involvement of Combinatorial Peptide Library Technique.International journal of molecular sciences · 2023Review
- Quantitative NMR Methods in Metabolomics.Handbook of experimental pharmacology · 2023Article
- Nuclear magnetic resonance spectroscopy of biofluids for osteoarthritis.British medical bulletin · 2021Article
- Metabolite Profile of Xylem Sap in Cotton Seedlings Is Changed by K Deficiency.Frontiers in plant science · 2020Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Synovial fluid (SF) is of great interest for the investigation of orthopedic pathologies, as it is in close proximity to various tissues that are primarily altered during these disease processes and can be collected using minimally invasive protocols. Multi-"omic" approaches are commonplace, although little consideration is often given for multiple analysis techniques at sample collection. Nuclear magnetic resonance (NMR) metabolomics and liquid chromatography tandem mass spectrometry (LC-MS/MS) proteomics are two complementary techniques particularly suited to the study of SF. However, currently there are no agreed upon standard protocols that are published for SF collection and processing for use with NMR metabolomic analysis. Furthermore, the large protein concentration dynamic range present within SF can mask the detection of lower abundance proteins in proteomics. While combinational ligand libraries (ProteoMiner columns) have been developed to reduce this dynamic range, their reproducibility when used in conjunction with SF, or on-bead protein digestion protocols, has yet to be investigated. Here we employ optimized protocols for the collection, processing, and storage of SF for NMR metabolite analysis and LC-MS/MS proteome analysis, including a Lys-C endopeptidase digestion step prior to tryptic digestion, which increased the number of protein identifications and improved reproducibility for on-bead ProteoMiner digestion.
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Registered trials
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