Evidence map›Paper›PMID 32226250›Full record

ArticleIndian journal of clinical biochemistry : IJCB2020

Effects of Different Concentrations of Reversine on Plasticity of Mesenchymal Stem Cells.

Leila Soltani, Hamid Reza Rahmani, Morteza Daliri Joupari, Hori Ghaneialvar, Amir Hossein Mahdavi, Mehdi Shamsara

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Indian journal of clinical biochemistry : IJCB, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 7 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Leila Soltani1Department of Animal Sciences, College of Agriculture, Isfahan University of Technology, Isfahan, Iran.
Hamid Reza Rahmani1Department of Animal Sciences, College of Agriculture, Isfahan University of Technology, Isfahan, Iran.
Morteza Daliri Joupari3Department of Animal Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Hori Ghaneialvar4Biotechnology and Medicinal Plants Research Center, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
Amir Hossein Mahdavi1Department of Animal Sciences, College of Agriculture, Isfahan University of Technology, Isfahan, Iran.
Mehdi Shamsara6National Center for Transgenic Mouse Research, Institute of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Isfahan University of Technology · IRNational Institute of Genetic Engineering and Biotechnology · IRMedical University of Ilam · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Dedifferentiation can be induced by small molecules. One of these small molecules used in this study in order to increase the plasticity of differentiation of stem cells was reversine. The objective of present study was to investigate the effect of different concentrations of reversine on the plasticity of ovine fetal bone-marrow mesenchymal stem cells (BM-MSCs). BM-MSCs were extracted from ovine fetal and cultured. Passaged-3 cells were evaluated for their differentiation potential into osteocytes and adipocytes cells. In the present study, BM-MSCs were culture plated in the presence of 0, 300, 600, 900 and 1200 nM of reversine. The number of viable cells was determined by MTT test after addition of different concentrations of reversine. Furthermore, expression of the nanog gene was evaluated. The culture without reversine was taken as the control group. Expression of nanog was analysed by immunocytochemistry. Multi-lineage differentiation showed that the BM-MSCs could be differentiated into adipose cells and osteocytes. Our results indicated that the addition of 1200 nM of reversine to medium significantly decreased overall proliferation compared to the other treatment groups (

Indexed as

Mesenchymal stem cellsOvine fetalPlasticityProliferationReversine

Identifiers

PMID32226250
PMCPMC7093655
OpenAlexW2904504262

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.