ArticleOptics express2020
Variable incidence angle linear dichroism (VALiD): a technique for unique 3D orientation measurement of fluorescent ensembles.
Article in Optics express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Volumetric imaging of the 3D orientation of cellular structures with a polarized fluorescence light-sheet microscope.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Illuminating cellular architecture and dynamics with fluorescence polarization microscopy.Journal of cell science · 2024Review
- Three-dimensional spatio-angular fluorescence microscopy with a polarized dual-view inverted selective-plane illumination microscope (pol-diSPIM).bioRxiv : the preprint server for biology · 2024Article
- DNA Nanotechnology for Investigating Mechanical Signaling in the Immune System.Angewandte Chemie (International ed. in English) · 2023Review
- Defining domain-specific orientational order in the desmosomal cadherins.Biophysical journal · 2022Article
- Photon efficient orientation estimation using polarization modulation in single-molecule localization microscopy.Biomedical optics express · 2022Article
- Turn-key mapping of cell receptor force orientation and magnitude using a commercial structured illumination microscope.Nature communications · 2021Article
- Live-cell super-resolved PAINT imaging of piconewton cellular traction forces.Nature methods · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
A fundamental challenge with fluorophore orientation measurement is degeneracy, which is the inability to distinguish between multiple unique fluorophore orientations. Techniques exist for the non-degenerate measurement of the orientations of single, static fluorophores. However, such techniques are unsuitable for densely labeled and/or dynamic samples common to biological research. Accordingly, a rapid, widefield microscopy technique that can measure orientation parameters for ensembles of fluorophores in a non-degenerate manner is desirable. We propose that exciting samples with polarized light and multiple incidence angles could enable such a technique. We use Monte Carlo simulations to validate this approach for specific axially symmetric ensembles of fluorophores and obtain optimal experimental parameters for its future implementation.
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Registered trials
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