ArticleJournal of visualized experiments : JoVE2020
Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts.
Article in Journal of visualized experiments : JoVE, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- LncRNA SNHG8 contributes to post-myocardial infarction cardiac fibrosis and is functionally associated with miR-192-3p.Journal of molecular histology · 2026Article
- Establishing Primary Cultures of Adult Mouse Cardiac Fibroblasts: A Comparative Evaluation of Three Experimental Protocols.International journal of molecular sciences · 2026Article
- ANTXR1 blockade enhances cardiac function in preclinical models of heart failure.Nature cardiovascular research · 2025Article
- Review
- Upregulated FoxO1 promotes arrhythmogenesis in mice with heart failure and preserved ejection fraction.Nature communications · 2025Article
- Assessment of the alpha 7 nicotinic acetylcholine receptor as an imaging marker of cardiac repair-associated processes using NS14490.EJNMMI research · 2024Article
- Fibroblast Reprogramming in Cardiac Repair.JACC. Basic to translational science · 2024Review
- Transfer of cardiomyocyte-derived extracellular vesicles to neighboring cardiac cells requires tunneling nanotubes during heart development.Theranostics · 2024Article
- Myocardial fibrosis and calcification are attenuated by microRNA-129-5p targeting Asporin and Sox9 in cardiac fibroblasts.JCI insight · 2023Article
- Proteomic Insights into Cardiac Fibrosis: From Pathophysiological Mechanisms to Therapeutic Opportunities.Molecules (Basel, Switzerland) · 2022Review
- Interaction of ARRDC4 With GLUT1 Mediates Metabolic Stress in the Ischemic Heart.Circulation research · 2022Article
- MBNL1 drives dynamic transitions between fibroblasts and myofibroblasts in cardiac wound healing.Cell stem cell · 2022Article
- Characterization, isolation, and in vitro culture of leptomeningeal fibroblasts.Journal of neuroimmunology · 2021Article
- Matricellular Protein Cilp1 Promotes Myocardial Fibrosis in Response to Myocardial Infarction.Circulation research · 2021Article
- Glycolysis Inhibition Alleviates Cardiac Fibrosis After Myocardial Infarction by Suppressing Cardiac Fibroblast Activation.Frontiers in cardiovascular medicine · 2021Article
- Proarrhythmic Electrical Remodeling by Noncardiomyocytes at Interfaces With Cardiomyocytes Under Oxidative Stress.Frontiers in physiology · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Cardiac fibrosis in response to injury is a physiological response to wound healing. Efforts have been made to study and target fibroblast subtypes that mitigate fibrosis. However, fibroblast research has been hindered due to the lack of universally acceptable fibroblast markers to identify quiescent as well as activated fibroblasts. Fibroblasts are a heterogenous cell population, making them difficult to isolate and characterize. The presented protocol describes three different methods to enrich fibroblasts and myofibroblasts from uninjured and injured mouse hearts. Using a standard and reliable protocol to isolate fibroblasts will enable the study of their roles in homeostasis as well as fibrosis modulation.
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Registered trials
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