Evidence map›Paper›PMID 32222779›Full record

ArticleArchives of microbiology2020

Non-pathogenic Escherichia coli biofilms: effects of growth conditions and surface properties on structure and curli gene expression.

James Leech, Stacey Golub, Wendy Allan, Mark J H Simmons, Tim W Overton

Open access · hybridAbstract read
In one paragraph

Article in Archives of microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 24 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Adhesion ofInternational journal of molecular sciences · 2023
    Article
  6. Article
  7. Article
  8. Article
  9. Cluster analysis and geospatial mapping of antibiotic resistantOne health (Amsterdam, Netherlands) · 2022
    Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

James LeechSchool of Chemical Engineering, College of Engineering and Physical Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
Stacey GolubSchool of Chemical Engineering, College of Engineering and Physical Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.ORCID http://orcid.org/0000-0001-5146-7346
Wendy AllanSchool of Chemical Engineering, College of Engineering and Physical Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
Mark J H SimmonsSchool of Chemical Engineering, College of Engineering and Physical Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.ORCID http://orcid.org/0000-0002-0655-3744
Tim W OvertonSchool of Chemical Engineering, College of Engineering and Physical Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK. t.w.overton@bham.ac.uk.ORCID http://orcid.org/0000-0003-3050-2549
University of Birmingham · GBUniversity of Kent · GB

Funding

Biotechnology and Biological Sciences Research Council CASE PhD studentshipBiotechnology and Biological Sciences Research Council PhD studentship
6 · The paper itself

Abstract

Biofilm formation is a harmful phenomenon in many areas, such as in industry and clinically, but offers advantages in the field of biocatalysis for the generation of robust biocatalytic platforms. In this work, we optimised growth conditions for the production of Escherichia coli biofilms by three strains (PHL644, a K-12 derivative with enhanced expression of the adhesin curli; the commercially-used strain BL21; and the probiotic Nissle 1917) on a variety of surfaces (plastics, stainless steel and PTFE). E. coli PHL644 and PTFE were chosen as optimal strain and substratum, respectively, and conditions (including medium, temperature, and glucose concentration) for biofilm growth were determined. Finally, the impact of these growth conditions on expression of the curli genes was determined using flow cytometry for planktonic and sedimented cells. We reveal new insights into the formation of biofilms and expression of curli in E. coli K-12 in response to environmental conditions.

Indexed as

Environmental ExposureAdhesins, BacterialBacterial AdhesionBacterial ProteinsBiofilmsEscherichia coliEscherichia coli ProteinsPlasticsPolytetrafluoroethyleneStainless SteelSurface PropertiesAdhesins, BacterialBacterial ProteinsCrl protein, BacteriaEscherichia coli ProteinsPlasticsPolytetrafluoroethyleneStainless SteelBiofilmConfocal laser scanning microscopyCurliOsmolarity

Identifiers

PMID32222779
PMCPMC7355273
OpenAlexW3013753984

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.