Evidence map›Paper›PMID 32222540›Full record

ArticleAnalytical biochemistry2020

Identifying transglutaminase reaction products via mass spectrometry as exemplified by the MUC2 mucin - Pitfalls and traps.

Liisa Arike, Gunnar C Hansson, Christian V Recktenwald

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical biochemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. The role of goblet cells and mucus in intestinal homeostasis.Nature reviews. Gastroenterology & hepatology · 2022
    Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Liisa ArikeDepartment of Medical Biochemistry, University of Gothenburg, Gothenburg, Sweden.
Gunnar C HanssonDepartment of Medical Biochemistry, University of Gothenburg, Gothenburg, Sweden.
Christian V RecktenwaldDepartment of Medical Biochemistry, University of Gothenburg, Gothenburg, Sweden. Electronic address: christian.recktenwald@medkem.gu.se.
University of Gothenburg · SE

Funding

The MUC2 Mucus Gel as an Innate Immune Mechanism that Inhibits InflammationU01AI095473 · NIAID · GOTEBORG UNIVERSITY · PI HANSSON, GUNNAR C., JOHANSSON, MALIN E.V. · 2011 to 2020
$2.6M
NIAID NIH HHS U01 AI095473
6 · The paper itself

Abstract

In order to demonstrate transglutaminase activity in biological samples immunological as well as glutamine- and amine-donor based assays are commonly used. However, the identification of the transglutaminase reaction product, i. e. the isopeptide cross-linked peptides/proteins or the deamidated protein/peptide are often neglected. This article describes a workflow for the detection of the products of transglutaminase-catalyzed reactions. In particular, possible pitfalls and traps that can arise during the mass spectrometry-based identification of isopeptide cross-links are addressed and characterised on actual samples.

Indexed as

BiocatalysisCross-Linking ReagentsMass SpectrometryMucin-2PeptidesTransglutaminasesCross-Linking ReagentsMUC2 protein, humanMucin-2PeptidesTransglutaminasesCross-linkIsopeptide bondMUC2MucusTransglutaminase

Identifiers

PMID32222540
PMCPMC7184670
OpenAlexW3014082444

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.