ArticleTicks and tick-borne diseases2020
Identification and validation of internal reference genes for real-time quantitative polymerase chain reaction-based studies in Hyalomma anatolicum ticks.
Article in Ticks and tick-borne diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 7 citations in OpenAlex.
- Improving Molecular Detection of Tick-Borne Pathogens in Citizen-Collected Ticks.Pathogens (Basel, Switzerland) · 2026Article
- Copy Number Variation-Based Molecular Sexing ofbioRxiv : the preprint server for biology · 2025Article
- Stable internal reference genes for quantitative RT-PCR analyses in Rhipicephalus microplus during embryogenesis.Ticks and tick-borne diseases · 2023Article
- The molecular and functional characterization of ferritins in the hard tick Hyalomma rufipes.Parasites & vectors · 2022Article
- Virus-Derived DNA Forms Mediate the Persistent Infection of Tick Cells by Hazara Virus and Crimean-Congo Hemorrhagic Fever Virus.Journal of virology · 2021Article
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Authors and funding
5 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Crimean-Congo hemorrhagic fever (CCHF) is an emerging tick-borne viral disease caused by the orthonairovirus CCHF virus (CCHFV). Ticks of the genus Hyalomma are the viral reservoir and they represent the main vector transmitting the virus to their hosts during blood feeding. However, how CCHFV replicates in its natural arthropod host cells and the nature of virus/host interactions are still largely unknown. With the aim of developing tools for use in this field, we identified and validated expression of four candidate endogenous control tick genes in a Hyalomma anatolicum-derived cell line. These genes will be useful for normalization of viral/cellular transcripts in infection/expression studies or as internal controls in molecular epidemiology surveys of pathogens transmitted by Hyalomma ticks.
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