ArticleMethods in molecular biology (Clifton, N.J.)2020
A Scalable Purification Method for Mitochondria from Trypanosoma brucei.
Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 8 citations in OpenAlex.
- Biochemistry of Diplonemids.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Isolation of Glycosomes from Trypanosoma brucei.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- Mitoribosomal small subunit maturation involves formation of initiation-like complexes.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- CTS tag-based methods for investigating mitochondrial RNA modification factors in Trypanosoma brucei.Methods in enzymology · 2021Article
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
Due to its unique biology the mitochondrion of Trypanosoma brucei has attracted a lot of interest since many decades, making it arguably the best studied mitochondrion outside yeast and mammals. Here we describe a method allowing purification of mitochondria from procyclic trypanosomes that yields highly enriched and functional organelles. The method is based on isotonic lysis of cells by nitrogen cavitation, DNase I digestion, differential centrifugation and Nycodenz gradient centrifugation. The method is scalable and can be adapted to culture volumes a small as 100 mL or as large as 24 L.
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