Evidence map›Paper›PMID 32221939›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2020

Gel-Based Methods for the Investigation of Signal Transduction Pathways in Trypanosoma brucei.

Balázs Szöőr, Mathieu Cayla

Open access · hybridAbstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Balázs Szöőr *Institute for Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh, UK. balazs.szoor@ed.ac.uk.
Mathieu Cayla *Institute for Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh, UK. Mathieu.Cayla@ed.ac.uk.
University of Edinburgh · GB

Funding

Wellcome Trust 103740/Z/14/ZWellcome Trust 103740/Z14/Z
6 · The paper itself

Abstract

In the cell, reversible phosphorylation, controlled by protein phosphatases and protein kinases, initiates and regulates various signaling-dependent processes such as enzyme-substrate interactions, the cell cycle, differentiation, and immune responses. In addition to these processes, in unicellular parasites like Trypanosoma brucei, the causative agent of African sleeping sickness, additional signaling pathways have evolved to enable the survival of parasites in the changing environment of the vector and mammalian host. In this chapter, we describe two in vitro kinase assays and the use of the phosphoprotein chelator Phos-tag and show that these three polyacrylamide gel-based assays can be used for rapid target validation and detection of changes in phosphorylation.

Indexed as

Chelating AgentsElectrophoresis, Polyacrylamide GelPhosphoproteinsPhosphorylationProtein KinasesProtozoan ProteinsPyridinesSignal TransductionStaining and LabelingTrypanosoma brucei brucei1,3-bis(bis(pyridin-2-ylmethyl)amino)propan-2-olChelating AgentsPhosphoproteinsProtein KinasesProtozoan ProteinsPyridinesKinase assaysPhospho substratePhos-tagProtein kinasesProtein phosphatasesSDS-PAGESignal transduction

Identifiers

PMID32221939
OpenAlexW3013262735

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.