Evidence map›Paper›PMID 32221937›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2020

All You Ever Wanted to Know About APOL1 and TLFs and Did Not Dare Ask.

Joseph Verdi, Charles Schaub, Russell Thomson, Jayne Raper

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
10.9field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Joseph VerdiHunter College, City University of New York, New York, NY, USA.
Charles SchaubHunter College, City University of New York, New York, NY, USA.
Russell ThomsonHunter College, City University of New York, New York, NY, USA.
Jayne RaperHunter College, City University of New York, New York, NY, USA. raper@genectr.hunter.cuny.edu.
Hunter College · USThe Graduate Center, CUNY · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Interest in trypanosome lytic factors (TLFs) and apolipoprotein L1, the ion channel-forming protein component of TLFs, has increased tenfold since 2010. This is due to the association of African variants of APOL1 with kidney disease such that interest has reached circles beyond parasitology. We have extensive experience purifying and working with these proteins and protein complexes. Herein we describe our detailed purification protocols to aid the new burgeoning field by providing an opportunity for consistency in reagents used across laboratories. We emphasize that it is imperative to maintain APOL1 protein intact (~42 kDa) to analyze the active ion channel-forming component/protein.

Indexed as

Apolipoprotein L1HumansKidney DiseasesLipoproteins, HDLMolecular WeightRecombinant ProteinsTrypanosomaTrypanosomiasis, AfricanAPOL1 protein, humanApolipoprotein L1Lipoproteins, HDLRecombinant ProteinsTLF1 protein, humanApolipoprotein L1Haptoglobin related proteinHigh density lipoproteinIon channelKidney diseasePlasmaSerumTrypanosome lytic factor

Identifiers

PMID32221937
OpenAlexW3013720882

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.