Evidence map›Paper›PMID 32221936›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2020

Airyscan Superresolution Microscopy to Study Trypanosomatid Cell Biology.

Jane Harmer, Asma Belbelazi, Martin Carr, Michael L Ginger

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Jane HarmerDepartment of Biological and Geographical Sciences, School of Applied Sciences, University of Huddersfield, Huddersfield, UK. J.Harmer@hud.ac.uk.
Asma BelbelaziDepartment of Biological and Geographical Sciences, School of Applied Sciences, University of Huddersfield, Huddersfield, UK.
Martin CarrDepartment of Biological and Geographical Sciences, School of Applied Sciences, University of Huddersfield, Huddersfield, UK.
Michael L GingerSchool of Applied Sciences, University of Huddersfield, Huddersfield, UK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The recent introduction by Carl Zeiss Ltd. of the Airyscan detector module for their LSM880 confocal laser-scanning microscope has enabled routine superresolution microscopy to be combined with the advantages of confocal-based fluorescence imaging. Resulting enhanced spatial resolution in X, Y, and Z provides tractable opportunity to derive new insight into protein localization(s), organelle dynamics, and thence protein function within trypanosomatids or other organisms. Here, we describe methods for preparing slides, cells, and basic microscope setup for fluorescence imaging of trypanosomatids using the LSM-880 with Airyscan platform.

Indexed as

CytoskeletonFlagellaFluorescent DyesIntravital MicroscopyMicroscopy, ConfocalStaining and LabelingTrypanosomatinaFluorescent DyesConfocalCrithidiaImmunofluorescenceLeishmaniaLive-cell imagingNovymonasSuperresolution microscopyTrypanosomaTrypanosomatids

Identifiers

PMID32221936

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.