Evidence map›Paper›PMID 32221930›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2020

Forward Genetics in African Trypanosomes.

Sebastian Hutchinson, Lucy Glover

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Sebastian HutchinsonINSERM, Unit U1201, Paris, France.
Lucy GloverTrypanosome Molecular Biology Group, Institut Pasteur, Paris, France. lucy.glover@pasteur.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Forward genetic screens in Trypanosoma brucei have enabled researchers to move from a candidate-gene based approach to one where we are able to studying all genes required for a single process simultaneously. In this protocol, we describe how to generate RNAi library strains in bloodstream form trypanosomes, run a screen by selecting for drug resistance or using a reporter gene and process the high-throughput sequencing data for a genome scale RNAi library screen.

Indexed as

Antiprotozoal AgentsCell LineDNA, ProtozoanDrug ResistanceGene LibraryGenes, ProtozoanGenes, ReporterHigh-Throughput Nucleotide SequencingHumansMolecular TypingProtozoan ProteinsRNA InterferenceSequence Analysis, DNATrypanosoma brucei bruceiTrypanosomiasis, AfricanAntiprotozoal AgentsDNA, ProtozoanProtozoan ProteinsForward geneticsHigh-throughput sequencingPhenotypic screenRNAiTrypanosoma brucei

Identifiers

PMID32221930

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.