ArticleMethods in molecular biology (Clifton, N.J.)2020
Identifying Trypanosome Protein-RNA Interactions Using RIP-Seq.
Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed, 14 citations in OpenAlex.
- Life stage-specific poly(A) site selection regulated byProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Selective nuclear export of mRNAs is promoted by DRBD18 in Trypanosoma brucei.Molecular microbiology · 2021Article
- A potential role for a novel ZC3H5 complex in regulating mRNA translation inThe Journal of biological chemistry · 2020Article
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2 authors at 2 institutions in 2 countries.
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Abstract
Trypanosomatids rely primarily on posttranscriptional mechanisms for the control of gene expression, with regulation of RNA processing, localization, degradation, and translation by RNA-binding proteins (RBPs). To determine the mechanisms by which RBPs control gene expression in trypanosomatids, transcriptome-wide identification of mRNA targets and mapping of the RNA-binding site is required. Here we present our most current RIP-Seq (RNA immunoprecipitation followed by high-throughput sequencing) protocol that we generally apply to elucidate RNA/protein interactions in Trypanosoma brucei. The technique provides valuable information about the workings of messenger ribonucleoprotein (mRNP) networks and trypanosome gene expression mechanisms.
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