Evidence map›Paper›PMID 32221924›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2020

Next-Generation Analysis of Trypanosomatid Genome Stability and Instability.

Emma M Briggs, Catarina A Marques, Joao Reis-Cunha, Jennifer Black, Samantha Campbell, Jeziel Damasceno, Daniella Bartholomeu, Kathryn Crouch, Richard McCulloch

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Emma M BriggsInstitute of Infection, Immunity and Inflammation, The Wellcome Centre for Integrative Parasitology, University of Glasgow, Glasgow, UK.
Catarina A MarquesInstitute of Infection, Immunity and Inflammation, The Wellcome Centre for Integrative Parasitology, University of Glasgow, Glasgow, UK.
Joao Reis-CunhaDepartamento de Parasitologia, Universidade Federal de Minas Gerais-Instituto de Ciências Biológicas, Belo Horizonte, Brazil.
Jennifer BlackInstitute of Infection, Immunity and Inflammation, The Wellcome Centre for Integrative Parasitology, University of Glasgow, Glasgow, UK.
Samantha CampbellInstitute of Infection, Immunity and Inflammation, The Wellcome Centre for Integrative Parasitology, University of Glasgow, Glasgow, UK.
Jeziel DamascenoInstitute of Infection, Immunity and Inflammation, The Wellcome Centre for Integrative Parasitology, University of Glasgow, Glasgow, UK.
Daniella BartholomeuDepartamento de Parasitologia, Universidade Federal de Minas Gerais-Instituto de Ciências Biológicas, Belo Horizonte, Brazil.
Kathryn CrouchInstitute of Infection, Immunity and Inflammation, The Wellcome Centre for Integrative Parasitology, University of Glasgow, Glasgow, UK.
Richard McCullochInstitute of Infection, Immunity and Inflammation, The Wellcome Centre for Integrative Parasitology, University of Glasgow, Glasgow, UK. richard.mcculloch@glasgow.ac.uk.

Funding

Biotechnology and Biological Sciences Research Council BB/K006495/1Biotechnology and Biological Sciences Research Council BB/M028909/1Biotechnology and Biological Sciences Research Council BB/N016165/1Medical Research Council G0401553Wellcome Trust 083485Wellcome Trust 089172Wellcome Trust 104111Wellcome Trust 206815
6 · The paper itself

Abstract

Understanding the rate and patterns of genome variation is becoming ever more amenable to whole-genome analysis through advances in DNA sequencing, which may, at least in some circumstances, have supplanted more localized analyses by cellular and genetic approaches. Whole-genome analyses can utilize both short- and long-read sequence technologies. Here we describe how sequence generated by these approaches has been used in trypanosomatids to examine DNA replication dynamics, the accumulation of modified histone H2A due to genome damage, and evaluation of genome variation, focusing on ploidy change.

Indexed as

Genomic InstabilityHigh-Throughput Nucleotide SequencingSequence Analysis, DNAChromosomesComputational BiologyDatasets as TopicDNA Copy Number VariationsDNA, ProtozoanGenome, ProtozoanHistonesLeishmania majorParasitologyDNA, ProtozoanHistonesChIP-seqCopy number variationDNA damageDNA replicationMFA-seqNext-generation sequencingPloidySingle nucleotide polymorphisms

Identifiers

PMID32221924

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.