Evidence map›Paper›PMID 32221789›Full record

ArticleJournal of assisted reproduction and genetics2020

The growth and development conditions in mouse offspring derived from ovarian tissue cryopreservation and orthotopic transplantation.

Zhe Yan, Qing Li, Long Zhang, Beijia Kang, Wei Fan, Tang Deng, Jiang Zhu, Yan Wang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.1field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Review
  3. Developing Mouse Models for Ovarian Tissue Transplantation and Xenotransplantation: A Review.Medical science monitor : international medical journal of experimental and clinical research · 2025
    Review
  4. Review
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Zhe YanDepartment of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu, 610041, China.ORCID https://orcid.org/0000-0002-2644-6794
Qing LiDepartment of Thoracic Oncology, West China hospital, Sichuan University, Chengdu, 610041, China.
Long ZhangReproductive Medical Center of West China 2nd University Hospital, Sichuan University, Ren Min Nan Lu, Chengdu, 610041, China.
Beijia KangReproductive Medical Center of West China 2nd University Hospital, Sichuan University, Ren Min Nan Lu, Chengdu, 610041, China.
Wei FanReproductive Medical Center of West China 2nd University Hospital, Sichuan University, Ren Min Nan Lu, Chengdu, 610041, China.
Tang DengReproductive Medical Center of West China 2nd University Hospital, Sichuan University, Ren Min Nan Lu, Chengdu, 610041, China.
Jiang ZhuDepartment of Thoracic Oncology, West China hospital, Sichuan University, Chengdu, 610041, China.
Yan WangReproductive Medical Center of West China 2nd University Hospital, Sichuan University, Ren Min Nan Lu, Chengdu, 610041, China. wangyy1210@163.com.ORCID https://orcid.org/0000-0003-2426-2542
Sichuan University · CN

Funding

Key Research and Development Program of Science and Technology department, Sichuan Province 2019YFS0418National Natural Science Foundation of China 31201117National Natural Science Foundation of China 81673032
6 · The paper itself

Abstract

purposeTo investigate the potential development or metabolic risk in offspring derived from mice with transplanted frozen-thawed ovarian tissue.

methodsMice ovaries were intervened by vitrification (group V) and slow-freezing (group S) cryopreservation and orthotopic transplantation. Orthotopic transplantation of fresh ovarian (group F) and natural mating (group C) served as control groups. The fertility restoration and health conditions of generations were assessed by offspring counts, anti-fatigue and motor ability, and organ morphology. The methylation rate and expression level of imprinted genes (IGF2R, H19, SNRPN, and PLAGL1) were used to predict the potential risk of development in transplanted generations.

resultsBoth the percentage of normal morphological follicles in different developmental periods and the litter size of receipt mice were comparable in all three transplanted groups. There was no significant difference in offspring mice's birth defects, body weight gain, anti-fatigue ability, or exercise capacity among the four groups. The methylation rate of IGF2R, H19, and PLAGL1 showed a significant variation in cryopreservation groups as compared with control groups, as well as a difference in gene expression. The SNRPN appeared to be stable in methylation status. There were no differences in mRNA expression in all groups.

conclusionsThe different ovarian tissue cryopreservation methods did not influence either maternal fertility function or offspring growth. However, these technologies could affect the methylation rate and expression level of some development-related imprinting genes in the offspring, which may lead to some indeterminacy risk.

Indexed as

CryopreservationGenomic ImprintingAnimalsFemaleFertilityFertility PreservationHumansMiceOvarian FollicleOvaryReproductionVitrificationFertility restorationGrowth and developmentImprinting genesOffspringOrthotopic transplantationOvarian tissue cryopreservation

Identifiers

PMID32221789
PMCPMC7183038
OpenAlexW3013132671

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.