Evidence map›Paper›PMID 32220451›Full record

ArticleJournal of the American College of Surgeons2020

Analysis of Plasma Products for Cellular Contaminants: Comparing Standard Preparation Methods.

Richard R Rieske, Matthew E Kutcher, Jon P Audia, Kristen T Carter, Yann-Leei Lee, Yong B Tan, Mark N Gillespie, Gina C Capley, Danielle M Tatum, Alison A Smith and 2 more

Abstract readComparative StudyMulticenter Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of the American College of Surgeons, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors.

Richard R RieskeDepartment of Surgery, The University of South Alabama, Mobile, AL.
Matthew E KutcherDepartment of Surgery, University of Mississippi, Jackson, MS.
Jon P AudiaDepartment of Microbiology and Immunology, The University of South Alabama, Mobile, AL.
Kristen T CarterDepartment of Surgery, University of Mississippi, Jackson, MS.
Yann-Leei LeeDepartment of Surgery, The University of South Alabama, Mobile, AL.
Yong B TanDepartment of Surgery, The University of South Alabama, Mobile, AL.
Mark N GillespieDepartment of Cellular and Molecular Pharmacology, The University of South Alabama, Mobile, AL.
Gina C CapleyDepartment of Cellular and Molecular Pharmacology, The University of South Alabama, Mobile, AL.
Danielle M TatumDepartment of Surgery, Our Lady of the Lake Regional Medical Center, Baton Rouge, LA.
Alison A SmithDepartment of Surgery, Tulane University, New Orleans, LA.
Juan C DuchesneDepartment of Surgery, Tulane University, New Orleans, LA.
Jon D SimmonsDepartment of Surgery, The University of South Alabama, Mobile, AL; Department of Cellular and Molecular Pharmacology, The University of South Alabama, Mobile, AL. Electronic address: jdsimmons@health.southalabama.edu.

Funding

Tracking and Evaluation CoreU54GM115428 · NIGMS · UNIVERSITY OF MISSISSIPPI MED CTR · PI COMPRETTA, CAROLINE E. · 2016 to 2025
$38.2M
UAB Center for Clinical and Translational Science (CCTS)UL1TR001417 · NCATS · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI KIMBERLY, ROBERT P. · 2015 to 2018
$31.4M
Role of obesity in preeclamptic pregnancy.P20GM121334 · NIGMS · UNIVERSITY OF MISSISSIPPI MED CTR · PI Noha Shawky · 2017 to 2026
$26.4M
mtDNA DAMPS: a pharmacologic target in multi-organ system failureK08GM109113 · NIGMS · UNIVERSITY OF SOUTH ALABAMA · PI SIMMONS, JON DAVID · 2016 to 2019
$756k
NCATS NIH HHS UL1 TR001417NIGMS NIH HHS K08 GM109113NIGMS NIH HHS P20 GM121334NIGMS NIH HHS U54 GM115428
6 · The paper itself

Abstract

backgroundRecent reports suggest that component plasma products contain significant quantities of cellular contamination. We hypothesized that leukoreduction of whole blood before preparation of derived plasma is an effective method to prevent cellular contamination of stored plasma. STUDY

designSamples of never-frozen liquid plasma prepared by standard methods (n = 25) were obtained from 3 regional blood centers that supply 3 major trauma centers. Samples were analyzed for leukocyte and platelet contamination by flow cytometry. To determine if leukoreduction of whole blood before centrifugation and expression of plasma prevents cellular contamination of liquid plasma, 1 site generated 6 additional units of liquid plasma from leukoreduced whole blood, which were then compared with units of liquid plasma derived by standard processing.

resultsAcross all centers, each unit of never-frozen liquid plasma contained a mean of 12.8 ± 3.0 million leukocytes and a mean of 4.6 ± 2 billion platelets. Introduction of whole blood leukoreduction (LR) before centrifugation and plasma extraction essentially eliminated all contaminating leukocytes (Non-LR: 12.3 ± 2.9 million vs LR: 0.05 ± 0.05 million leukocytes) and platelets (Non-LR: 4.2 ± 0.3 billion platelets vs LR: 0.00 ± 0.00 billion platelets).

conclusionsDespite widespread belief that stored plasma is functionally acellular, testing of liquid plasma from 3 regional blood banks revealed a significant amount of previously unrecognized cellular contamination. Introduction of a leukoreduction step before whole blood centrifugation essentially eliminated detectable leukocyte and platelet contaminants from plasma. Therefore, our study highlights a straightforward and cost-effective method to eliminate cellular contamination of stored plasma.

Indexed as

Blood PlateletsLeukocytesHumansLeukocyte Reduction ProceduresMalePlasma

Identifiers

PMID32220451
PMCPMC7682813

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.