ArticleAnnals of clinical microbiology and antimicrobials2020
Investigation of biofilm production and its association with genetic and phenotypic characteristics of OM (osteomyelitis) and non-OM orthopedic Staphylococcus aureus.
Article in Annals of clinical microbiology and antimicrobials, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed.
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- Characterization ofBiofilm · 2025Article
- Antibiotic Resistance and Molecular Characterization ofMicroorganisms · 2025Article
- Isolation ofInternational journal of microbiology · 2025Article
- Biofilm-producing ability of methicillin-resistant Staphylococcus aureus clinically isolated in China.BMC microbiology · 2024Article
- Review
- Molecular Evolution and Genomic Insights into Community-Acquired Methicillin-Resistant Staphylococcus aureus Sequence Type 88.Microbiology spectrum · 2022Article
- Surveillance of osteoarticular infections caused byFrontiers in cellular and infection microbiology · 2022Article
- Biofilm Formation of Multidrug-Resistant MRSA Strains Isolated from Different Types of Human Infections.Pathogens (Basel, Switzerland) · 2021Article
- The Prevalence ofAnimals : an open access journal from MDPI · 2021Article
- Promising Antibiofilm Agents: Recent Breakthrough against Biofilm Producing Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2020Article
- Review
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Abstract
backgroundStaphylococcus aureus is a primary pathogen of orthopedic infections. By mediating antimicrobial resistance, S. aureus biofilm plays an important role in the recalcitrance of orthopedic infections, especially for the intractable osteomyelitis (OM). This study investigated the relationship between biofilm production and various genetic or phenotypic characteristics among orthopedic S. aureus strains.
methodsA total of 137 orthopedic S. aureus isolates were enrolled and divided into OM and non-OM groups. Biofilm production was evaluated using the crystal violet assay. Genetic and phenotypic characteristics including MRSA identification, MLST and spa typing, carriage of virulence genes, drug resistance, and patients' inflammatory responses indicators were characterized. The relationship between biofilm production and above-mentioned features was respectively analyzed among all isolates and compared between OM and non-OM isolates.
resultsBiofilm production presented no significant difference between OM (including 9 MRSA isolates) and non-OM (including 21 MRSA isolates) strains. We found that ST88, t377 and ST630-MSSA-t377 strains produced very strong biofilms, while MLST types of ST15, ST25, ST398, ST5, ST59 and spa types of t002, t2325, t437 tended to produce weaker biofilms. Strains with the following profiles produced stronger biofilms: fib(+)-hlgv(+)-lukED(+)-sei(-)-sem(-)-seo(-) for all isolates, sei(-)-sem(-)-seo(-) for OM isolates, and cna (+)-fib (+)-hlgv (+)-lukED (+)-seb(-)-sed(-) for non-OM isolates. In addition, not any single drug resistance was found to be related to biofilm production. We also observed that, among OM patients, strains with stronger biofilms caused weaker inflammatory responses.
conclusionSome genetic or phenotypic characteristics of orthopedic strains were associated with biofilm production, and this association could be different among OM and non-OM strains. The results are of great significance for better understanding, evaluating and managing different kinds of biofilm-associated orthopedic infections, and provide potential targets for biofilm clearance.
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