Evidence map›Paper›PMID 32219913›Full record

ArticleProtein science : a publication of the Protein Society2020

Identification and in silico bioinformatics analysis of PR10 proteins in cashew nut.

Shanna Bastiaan-Net, Maria C Pina-Pérez, Bas J W Dekkers, Adrie H Westphal, Antoine H P America, Renata M C Ariëns, Nicolette W de Jong, Harry J Wichers, Jurriaan J Mes

Abstract read
In one paragraph

Article in Protein science : a publication of the Protein Society, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Current Insights on the Impact of Proteomics in Respiratory Allergies.International journal of molecular sciences · 2022
    Review
  9. Identification and in silico bioinformatics analysis of PR10 proteins in cashew nut.Protein science : a publication of the Protein Society · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Shanna Bastiaan-NetWageningen Food and Biobased Research, Wageningen University and Research, Wageningen, The Netherlands.ORCID 0000-0001-8922-7305
Maria C Pina-PérezInstitute of Life Technologies, HES-SO Valais-Wallis, Sion, Switzerland.
Bas J W DekkersWageningen Seed Lab, Laboratory of Plant Physiology, Wageningen University, Wageningen, The Netherlands.
Adrie H WestphalBiochemistry, Wageningen University and Research, Wageningen, The Netherlands.
Antoine H P AmericaWageningen Plant Research, Wageningen University and Research, Wageningen, The Netherlands.
Renata M C AriënsWageningen Food and Biobased Research, Wageningen University and Research, Wageningen, The Netherlands.
Nicolette W de JongAllergology, Department of Internal Medicine, Erasmus MC, Rotterdam, The Netherlands.
Harry J WichersWageningen Food and Biobased Research, Wageningen University and Research, Wageningen, The Netherlands.
Jurriaan J MesWageningen Food and Biobased Research, Wageningen University and Research, Wageningen, The Netherlands.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Proteins from cashew nut can elicit mild to severe allergic reactions. Three allergenic proteins have already been identified, and it is expected that additional allergens are present in cashew nut. pathogenesis-related protein 10 (PR10) allergens from pollen have been found to elicit similar allergic reactions as those from nuts and seeds. Therefore, we investigated the presence of PR10 genes in cashew nut. Using RNA-seq analysis, we were able to identify several PR10-like transcripts in cashew nut and clone six putative PR10 genes. In addition, PR10 protein expression in raw cashew nuts was confirmed by immunoblotting and liquid chromatography-mass spectrometry (LC-MS/MS) analyses. An in silico allergenicity assessment suggested that all identified cashew PR10 proteins are potentially allergenic and may represent three different isoallergens.

Indexed as

AllergensAnacardiumComputer SimulationNutsPlant ProteinsRNA-SeqChromatography, LiquidTandem Mass SpectrometryAllergensPlant ProteinsAnacardium occidentaleBet v 1-likecashew nutin silico allergenicity analysisoral allergy syndrome (OAS)PR10RNA-seq

Identifiers

PMID32219913
PMCPMC7314402

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.