ArticleIntegrative biology : quantitative biosciences from nano to macro2020
Endothelial cell crosstalk improves browning but hinders white adipocyte maturation in 3D engineered adipose tissue.
Article in Integrative biology : quantitative biosciences from nano to macro, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
- Fabricating Microfluidic Co-Cultures of Immortalized Cell Lines Uncovers Robust Design Principles for the Simultaneous Formation of Patterned, Vascularized, and Stem Cell-Derived Adipose Tissue.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- MEBOCOST maps metabolite-mediated intercellular communications using single-cell RNA-seq.Nucleic acids research · 2025Article
- BCR::ABL1 expression in chronic myeloid leukemia cells in low oxygen is regulated by glutamine via CD36-mediated fatty acid uptake.Cancer cell international · 2025Article
- Paracrine role of endothelial IGF-1 receptor in depot-specific adipose tissue adaptation in male mice.Nature communications · 2025Article
- Development and analysis of scaffold-free adipose spheroids.Adipocyte · 2024Article
- Review
- Cationic polymer effect on brown adipogenic induction of dedifferentiated fat cells.Materials today. Bio · 2024Article
- Enhanced Angiogenesis in HUVECs Preconditioned with Media from Adipocytes Differentiated from Lipedema Adipose Stem Cells In Vitro.International journal of molecular sciences · 2023Article
- Modeling lymphangiogenesis: Pairing in vitro and in vivo metrics.Microcirculation (New York, N.Y. : 1994) · 2023Review
- Adipose Cells Induce Escape from an Engineered Human Breast Microtumor Independently of their Obesity Status.Cellular and molecular bioengineering · 2023Article
- Improving Fat Transplantation Survival and Vascularization with Adenovirus E4+ Endothelial Cell-Assisted Lipotransfer.Cells, tissues, organs · 2023Article
- Development of a Vascularized Human Skin Equivalent with Hypodermis for Photoaging Studies.Biomolecules · 2022Article
- Towards better models for studying human adipocytesAdipocyte · 2022Article
- 3D bioprinted white adipose model forBiofabrication · 2022Article
- Bringing hydrogel-based craniofacial therapies to the clinic.Acta biomaterialia · 2022Review
- High-throughput drug screening models of mature adipose tissues which replicate the physiology of patients' Body Mass Index (BMI).Bioactive materials · 2022Article
- Hypoxia induces stress fiber formation in adipocytes in the early stage of obesity.Scientific reports · 2021Article
- Mechanical Memory Impairs Adipose-Derived Stem Cell (ASC) Adipogenic Capacity After Long-TermCellular and molecular bioengineering · 2021Article
- miRNA induced co-differentiation and cross-talk of adipose tissue-derived progenitor cells for 3D heterotypic pre-vascularized bone formation.Biofabrication · 2021Article
- Angiogenesis in Adipose Tissue: The Interplay Between Adipose and Endothelial Cells.Frontiers in physiology · 2020Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Central to the development of adipose tissue (AT) engineered models is the supporting vasculature. It is a key part of AT function and long-term maintenance, but the crosstalk between adipocytes and endothelial cells is not well understood. Here, we directly co-culture the two cell types at varying ratios in a 3D Type I collagen gel. Constructs were evaluated for adipocyte maturation and function and vascular network organization. Further, these constructs were treated with forskolin, a beta-adrenergic agonist, to stimulate lipolysis and browning. Adipocytes in co-cultures were found to be less mature than an adipocyte-only control, shown by smaller lipid droplets and downregulation of key adipocyte-related genes. The most extensive vascular network formation was found in the 1:1 co-culture, supported by vascular endothelial growth factor (VEGF) upregulation. After forskolin treatment, the presence of endothelial cells was shown to upregulate PPAR coactivator 1 alpha (PGC-1α) and leptin, but not uncoupling protein 1 (UCP1), suggesting a specific crosstalk that enhances early stages of browning.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.