ArticleNature communications2020
A pocket-escaping design to prevent the common interference with near-infrared fluorescent probes in vivo.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- pKa engineering of a near-infrared fluorescent probe for sensitive imaging of oxidative stress in atherosclerotic plaques.Smart molecules : open access · 2026Article
- In Situ Lipoprotein-seeking Dye for in Vivo Real-Time Imaging of Lipid Dysregulation Diseases.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Molecular Imaging Probes for Early Detection and Staging of Liver Fibrosis.Chemical & biomedical imaging · 2025Review
- Advances in Organic Small Molecule-Based Fluorescent Probes for Precision Detection of Liver Diseases: A Perspective on Emerging Trends and Challenges.Journal of the American Chemical Society · 2025Review
- Interpretable Artificial Intelligence Decodes the Chemical Structural Essence of Twisted Intramolecular Charge Transfer and Planar Intramolecular Charge Transfer Fluorophores.Research (Washington, D.C.) · 2025Article
- Monitoring Glutathione Content of the Endoplasmic Reticulum under Scrap Leather-Induced Endoplasmic Reticulum Stress via an Endoplasmic Reticulum-Targeted Two-Photon Fluorescent Probe.Analytical chemistry · 2024Article
- Albumin-seeking dyes with adjustable assembliesTheranostics · 2024Article
- The investigation of the ultrafast excited state deactivation mechanisms for coumarin 307 in different solvents.RSC advances · 2023Article
- Practical Guidance for Developing Small-Molecule Optical Probes for In Vivo Imaging.Molecular imaging and biology · 2023Review
- Comprehensive Effect of Carbon Tetrachloride and Reversal of Gandankang Formula in Mice Liver: Involved in Oxidative Stress, Excessive Inflammation, and Intestinal Microflora.Antioxidants (Basel, Switzerland) · 2022Article
- Super-stable cyanine@albumin fluorophore for enhanced NIR-II bioimaging.Theranostics · 2022Article
- NIR-II cell endocytosis-activated fluorescent probes forChemical science · 2021Article
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Authors and funding
8 authors.
Funding
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Abstract
Near-infrared (NIR) fluorescent probes are among the most attractive chemical tools for biomedical imaging. However, their in vivo applications are hindered by albumin binding, generating unspecific fluorescence that masks the specific signal from the analyte. Here, combining experimental and docking methods, we elucidate that the reason for this problem is an acceptor (A) group-mediated capture of the dyes into hydrophobic pockets of albumin. This pocket-capturing phenomenon commonly applies to dyes designed under the twisted intramolecular charge-transfer (TICT) principle and, therefore, represents a generic but previously unidentified backdoor problem. Accordingly, we create a new A group that avoids being trapped into the albumin pockets (pocket-escaping) and thereby construct a NIR probe, BNLBN, which effectively prevents this backdoor problem with increased imaging accuracy for liver fibrosis in vivo. Overall, our study explains and overcomes a fundamental problem for the in vivo application of a broad class of bioimaging tools.
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