ArticleMicroorganisms2020
Litterbox-A gnotobiotic Zeolite-Clay System to Investigate Arabidopsis-Microbe Interactions.
Article in Microorganisms, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it.
- From Microbial Dynamics to Functionality in the Rhizosphere: A Systematic Review of the Opportunities With Synthetic Microbial Communities.Frontiers in plant science · 2021Pooled it
- From the leaf to the gut and back again: using synthetic bacterial communities to trace the fate and influence of phyllosphere bacteria in an Arabidopsis-Pieris brassicae system.Environmental microbiome · 2026Article
- The Alkane 1-Monooxygenase Gene alkB of Pseudomonas sp. FF2 Is Upregulated During Colonisation of Arabidopsis thaliana Leaves.Environmental microbiology reports · 2025Article
- Correlation ofApplied and environmental microbiology · 2025Article
- Surfactin facilitates establishment of Bacillus subtilis in synthetic communities.The ISME journal · 2025Article
- Differential Responses of Methylobacterium and Sphingomonas Species to Multispecies Interactions in the Phyllosphere.Environmental microbiology · 2025Article
- Beyond correlation: Understanding the causal link between microbiome and plant health.Heliyon · 2024Review
- Hitching a Ride in the Phyllosphere: Surfactant Production of Pseudomonas spp. Causes Co-swarming of Pantoea eucalypti 299R.Microbial ecology · 2024Article
- Nonpathogenic leaf-colonizing bacteria elicit pathogen-like responses in a colonization density-dependent manner.Plant-environment interactions (Hoboken, N.J.) · 2024Article
- Fabricated devices for performing bacterial-fungal interaction experiments across scales.Frontiers in microbiology · 2024Review
- Establishment of a transparent soil system to study Bacillus subtilis chemical ecology.ISME communications · 2023Article
- Metabolic resource overlap impacts competition among phyllosphere bacteria.The ISME journal · 2023Article
- Biosurfactants Produced by Phyllosphere-Colonizing Pseudomonads Impact Diesel Degradation but Not Colonization of Leaves of Gnotobiotic Arabidopsis thaliana.Applied and environmental microbiology · 2021Article
- Gnotobiotic evaluation of Dalbergia sissoo genotypes for resistance against Fusarium solani via dual culture set up.Journal, genetic engineering & biotechnology · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Plants are colonised by millions of microorganisms representing thousands of species withvarying effects on plant growth and health. The microbial communities found on plants arecompositionally consistent and their overall positive effect on the plant is well known. However,the effects of individual microbiota members on plant hosts and vice versa, as well as the underlyingmechanisms, remain largely unknown. Here, we describe "Litterbox", a highly controlled system toinvestigate plant-microbe interactions. Plants were grown gnotobiotically, otherwise sterile, onzeolite-clay, a soil replacement that retains enough moisture to avoid subsequent watering.Litterbox-grown plants resemble greenhouse-grown plants more closely than agar-grown plantsand exhibit lower leaf epiphyte densities (106 cfu/g), reflecting natural conditions. Apolydimethylsiloxane (PDMS) sheet was used to cover the zeolite, significantly lowering thebacterial load in the zeolite and rhizosphere. This reduced the likelihood of potential systemicresponses in leaves induced by microbial rhizosphere colonisation. We present results of exampleexperiments studying the transcriptional responses of leaves to defined microbiota members andthe spatial distribution of bacteria on leaves. We anticipate that this versatile and affordable plantgrowth system will promote microbiota research and help in elucidating plant-microbe interactionsand their underlying mechanisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.