ArticleNanomaterials (Basel, Switzerland)2020
Carbon Dots for Intracellular pH Sensing with Fluorescence Lifetime Imaging Microscopy.
Article in Nanomaterials (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Stable nitrogen-doped carbon quantum dots with pH-controlled fluorescence response for FeScientific reports · 2026Article
- Novel Carbon Dots Nanomaterials for the Precision Diagnosis and Treatment of Acute Lung Injury and Acute Respiratory Distress Syndrome: Mechanisms and Applications.International journal of nanomedicine · 2026Review
- Denoising of fluorescence lifetime imaging data via principal component analysis.Scientific reports · 2025Article
- Carbon dots as probes in FLIM:a review of applications and advances in cellular imaging.RSC advances · 2025Review
- Hollow NCQDs@HSiOJournal of fluorescence · 2025Article
- Development of Multifunctional C-dots from Processing Waste of Pomelo Fruits for Determination of pH and Methyl Parathion.Journal of fluorescence · 2025Article
- Ratiometric Fluorescent pH Sensing with Carbon Dots: Fluorescence Mapping across pH Levels for Potential Underwater Applications.Nanomaterials (Basel, Switzerland) · 2024Article
- Advances in Carbon Dot-Based Ratiometric Fluorescent Probes for Environmental Contaminant Detection: A Review.Micromachines · 2024Review
- Probing Polarity and pH Sensitivity of Carbon Dots inNanomaterials (Basel, Switzerland) · 2023Article
- Fluorescent Carbon Dots from Food Industry By-Products for Cell Imaging.Journal of functional biomaterials · 2023Article
- Carbon dot-based nanomaterials: a promising future nano-platform for targeting tumor-associated macrophages.Frontiers in immunology · 2023Review
- Ratiometric intracellular pH sensors based on nitrogen-doped graphene oxide quantum dots.Heliyon · 2022Article
- A Novel Quantum Dot-Based pH Probe for Long-Term Fluorescence Lifetime Imaging Microscopy Experiments in Living Cells.ACS applied materials & interfaces · 2022Article
- Visual pH Sensors: From a Chemical Perspective to New Bioengineered Materials.Molecules (Basel, Switzerland) · 2021Review
- Carbon Nanomaterials: Synthesis, Functionalization and Sensing Applications.Nanomaterials (Basel, Switzerland) · 2021Review
- Graphene Quantum Dots as Intracellular Imaging-Based Temperature Sensors.Materials (Basel, Switzerland) · 2021Article
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8 authors.
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Abstract
The monitoring of intracellular pH is of great importance for understanding intracellular trafficking and functions. It has various limitations for biosensing based on the fluorescence intensity or spectra study. In this research, pH-sensitive carbon dots (CDs) were employed for intracellular pH sensing with fluorescence lifetime imaging microscopy (FLIM) for the first time. FLIM is a highly sensitive method that is used to detect a microenvironment and it can overcome the limitations of biosensing methods based on fluorescence intensity. The different groups on the CDs surfaces changing with pH environments led to different fluorescence lifetime values. The CDs aqueous solution had a gradual change from 1.6 ns to 3.7 ns in the fluorescence lifetime with a pH range of 2.6-8.6. Similar fluorescence lifetime changes were found in pH buffer-treated living cells. The detection of lysosomes, cytoplasm, and nuclei in living cells was achieved by measuring the fluorescence lifetime of CDs. In particular, a phasor FLIM analysis was used to improve the pH imaging. Moreover, the effects of the coenzymes, amino acids, and proteins on the fluorescence lifetime of CDs were examined in order to mimic the complex microenvironment inside the cells.
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