Evidence map›Paper›PMID 32218171›Full record

ArticleInternational journal of molecular sciences2020

Highly Efficient Protoplast Isolation and Transient Expression System for Functional Characterization of Flowering Related Genes in

Rui Ren, Jie Gao, Chuqiao Lu, Yonglu Wei, Jianpeng Jin, Sek-Man Wong, Genfa Zhu, Fengxi Yang

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
3.3field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 60 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Establishment ofPlants (Basel, Switzerland) · 2025
    Article
  6. Beyond a few bases: methods for large DNA insertion and gene targeting in plants.The Plant journal : for cell and molecular biology · 2025
    Review
  7. Article
  8. Article
  9. Review
  10. Article
  11. Review
  12. Advances inFrontiers in plant science · 2024
    Article
  13. Article
  14. Review
  15. Article
  16. Article
  17. Optimization of Isolation and Transformation of Protoplasts fromInternational journal of molecular sciences · 2023
    Article
  18. Functional conservation and divergence ofFrontiers in plant science · 2023
    Article
  19. Article
  20. Embryogenic Stem Cell Identity after Protoplast Isolation fromInternational journal of molecular sciences · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Rui RenGuangdong Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Jie GaoGuangdong Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Chuqiao LuGuangdong Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Yonglu WeiGuangdong Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Jianpeng JinGuangdong Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Sek-Man WongDepartment of Biological Sciences, National University of Singapore (NUS), 14 Science Drive 4, Singapore 117543, Singapore.
Genfa ZhuGuangdong Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Fengxi YangGuangdong Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Guangdong Academy of Agricultural Sciences · CNTemasek Life Sciences Laboratory · SG

Funding

Chinese postdoctoral science foundation 2018M643033Discipline team Building projects of Guangdong Academy of Agricultural Sciences in the 13th Five-Year Period 201612TDGuangdong academy of agricultural sciences project 201612TD, R2016PY-QF015, 2017A070702008, President fund 201721, 201804bGuangdong modern agricultural industrial technology system flower innovation team expert project 2019KJ121Guangzhou scientific research project 201904020026, 201707010307National natural science foundation of China 31672184,31902065Natural Science Foundation of Guangdong province 2017A030312004R&D plan in key areas of Guangdong Province 2018B020202001Special fund for scientific innovation strategy-construction of high level Academy of Agriculture Science R2016PY-QF015the National Key Technologies R & D Program 2019YFD1001003, 2018YFD1000404
6 · The paper itself

Abstract

Protoplast systems have been proven powerful tools in modern plant biology. However, successful preparation of abundant viable protoplasts remains a challenge for

Indexed as

Cell SeparationFlowersGene Expression Regulation, PlantOrchidaceaePlant ProteinsProtein BindingProtoplastsPlant ProteinsCymbidium orchids, protoplast isolation, protoplast-based transient expression system (PTES), subcellular location, bimolecular fluorescence complementation (BiFC) assay, gene regulation

Identifiers

PMID32218171
PMCPMC7177621
OpenAlexW3013430815

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.