Evidence map›Paper›PMID 32217990›Full record

ArticleJournal of the Chinese Medical Association : JCMA2020

Enhancing induced pluripotent stem cell toward differentiation into functional cardiomyocytes.

Chian-Shiu Chien, Chien-Ying Wang, Hsin-Bang Leu, Yueh Chien, Yi-Ping Yang, Chia-Lin Wang, Hsiao-Yun Tai, Yu-Ling Ko, Fu-Ting Tsai, Shih-Jie Chou and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of the Chinese Medical Association : JCMA, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Chian-Shiu ChienDepartment of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan, ROC.
Chien-Ying WangFaculty of Medicine, National Yang-Ming University, Taipei, Taiwan, ROC.
Hsin-Bang LeuDepartment of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan, ROC.
Yueh ChienDepartment of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan, ROC.
Yi-Ping YangDepartment of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan, ROC.
Chia-Lin WangDepartment of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan, ROC.
Hsiao-Yun TaiDepartment of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan, ROC.
Yu-Ling KoDepartment of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan, ROC.
Fu-Ting TsaiDepartment of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan, ROC.
Shih-Jie ChouDepartment of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan, ROC.
Wen-Chung YuFaculty of Medicine, National Yang-Ming University, Taipei, Taiwan, ROC.
Meng-Yin YangDepartment of Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taichung, Taiwan, ROC.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHeart diseases, especially myocardial ischemia, remain one of the leading causes of mortality worldwide and usually result in irreparable cardiomyocyte damage and severe heart failure. Recent advances in induced pluripotent stem cell (iPSC) technologies for applied regenerative medicine and stem cell research, especially for iPSC-derived cardiomyocytes have increased the hope for heart repair. However, the driver molecules of myocardial differentiation and the functional reconstruction capacity of iPSC-derived cardiomyocytes are still questionable.

methodsHerein, we established a rapid differentiated platform that is involved in cardiomyogenic differentiation and maturation from iPSCs in vitro. Functional analysis is performed in miR-181a-transfected iPSC-derived cardiomyocyte (iPSC-cardio/miR-181a) under a time-lapse microscope. In addition, we calculated the beating area and frequency of iPSC-cardio/miR-181a cells in the presence of HCN4 shRNA or miR-181a SPONGE.

resultsmiR-181a enhanced the beating area and maintained the beating frequency of iPSC-derived cardiomyocytes by enhancing HCN4 expression.

conclusionmiR-181a would play a key role on maintaining proper beating function in iPSC-derived cardiomyocytes.

Indexed as

AnimalsCell DifferentiationCells, CulturedHyperpolarization-Activated Cyclic Nucleotide-Gated ChannelsInduced Pluripotent Stem CellsMiceMice, Inbred C57BLMicroRNAsMyocytes, CardiacHcn4 protein, mouseHyperpolarization-Activated Cyclic Nucleotide-Gated ChannelsMicroRNAsmirn181 microRNA, mouse

Identifiers

PMID32217990
PMCPMC12999157

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.