ArticleeLife2020
A calibrated optogenetic toolbox of stable zebrafish opsin lines.
Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed.
- CLOK: a chemigenetic multicolor labeling system to visualize neuronal birthdate and circuit integration.Nature neuroscience · 2026Article
- Decay chemicals activate a pH-sensing pathway to initiate a persistent state.bioRxiv : the preprint server for biology · 2026Article
- Spinal circuits encode a map of the trunk to control skin twitches.bioRxiv : the preprint server for biology · 2026Article
- Review
- Signal control during tissue regeneration in adult animals.Nature reviews. Molecular cell biology · 2026Review
- Synchronization of behavioral and cardiac dynamics in larval zebrafish.Cell reports · 2026Article
- Myelin sheaths in the central nervous system can withstand damage and dynamically remodel.Science (New York, N.Y.) · 2026Article
- Inwardly rectifying potassium channels promote directional sensing during neutrophil chemotaxis.The Journal of cell biology · 2026Article
- Optogenetic interrogation of the zebrafish lateral line reveals brain-wide neural circuits involved in pattern separation.iScience · 2025Article
- Optogenetic silencing by combining a rhodopsin cyclase with an engineered cGMP-gated potassium channel.Science advances · 2025Article
- Activity-driven myelin sheath growth is mediated by mGluR5.Nature neuroscience · 2025Article
- Inwardly rectifying potassium channels regulate membrane potential polarization and direction sensing during neutrophil chemotaxis.bioRxiv : the preprint server for biology · 2025Article
- Ketamine induces plasticity in a norepinephrine-astroglial circuit to promote behavioral perseverance.Neuron · 2025Article
- Subsets of extraocular motoneurons produce kinematically distinct saccades during hunting and exploration.Current biology : CB · 2025Article
- Transsynaptic labeling and transcriptional control of zebrafish neural circuits.Nature neuroscience · 2025Article
- Two-photon all-optical neurophysiology for the dissection of larval zebrafish brain functional and effective connectivity.Communications biology · 2024Article
- Neuropeptidergic regulation of neuromuscular signaling in larval zebrafish alters swimming behavior and synaptic transmission.iScience · 2024Article
- Closing the Experiment-Modeling-Perturbation Loop in Whole-Brain Neuroscience.Neuroscience bulletin · 2024Article
- All-optical interrogation of brain-wide activity in freely swimming larval zebrafish.iScience · 2024Article
- Diving into the zebrafish brain: exploring neuroscience frontiers with genetic tools, imaging techniques, and behavioral insights.Frontiers in molecular neuroscience · 2024Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
Optogenetic actuators with diverse spectral tuning, ion selectivity and kinetics are constantly being engineered providing powerful tools for controlling neural activity with subcellular resolution and millisecond precision. Achieving reliable and interpretable in vivo optogenetic manipulations requires reproducible actuator expression and calibration of photocurrents in target neurons. Here, we developed nine transgenic zebrafish lines for stable opsin expression and calibrated their efficacy in vivo. We first used high-throughput behavioural assays to compare opsin ability to elicit or silence neural activity. Next, we performed in vivo whole-cell electrophysiological recordings to quantify the amplitude and kinetics of photocurrents and test opsin ability to precisely control spiking. We observed substantial variation in efficacy, associated with differences in both opsin expression level and photocurrent characteristics, and identified conditions for optimal use of the most efficient opsins. Overall, our calibrated optogenetic toolkit will facilitate the design of controlled optogenetic circuit manipulations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.