Evidence map›Paper›PMID 32216371›Full record

ArticleACS synthetic biology2020

Efficient and Reproducible Multigene Expression after Single-Step Transfection Using Improved BAC Transgenesis and Engineering Toolkit.

Binhui Zhao, Pankaj Chaturvedi, David L Zimmerman, Andrew S Belmont

Open access · greenAbstract read
In one paragraph

Article in ACS synthetic biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.3field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Review
  3. The Regenerative Power of Stem Cells: Treating Bleomycin-Induced Lung Fibrosis.Stem cells and cloning : advances and applications · 2023
    Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Binhui ZhaoDepartment of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.ORCID 0000-0001-9390-3660
Pankaj ChaturvediDepartment of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.ORCID 0000-0001-7596-2165
David L ZimmermanDepartment of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.
Andrew S BelmontDepartment of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.ORCID 0000-0002-6540-0801
University of Illinois Urbana-Champaign · US

Funding

Mapping Technology DevelopmentU54DK107965 · NIDDK · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI BELMONT, ANDREW STEVEN · 2015 to 2019
$8.6M
CHROMATIN DOMAIN STRUCTURE/FUNCTIONR01GM058460 · NIGMS · UNIVERSITY OF ILLINOIS URBANA-CHAMPAIGN · PI BELMONT, ANDREW STEVEN · 1999 to 2025
$8.0M
LARGE SCALE CHROMATIN STRUCTURE/FUNCTIONR01GM042516 · NIGMS · UNIVERSITY OF ILLINOIS URBANA-CHAMPAIGN · PI BELMONT, ANDREW STEVEN · 1995 to 2007
$1.9M
Engineering stable, independent multi-transgene expression in mammalian cellsR01GM098319 · NIGMS · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI BELMONT, ANDREW STEVEN · 2011 to 2014
$1.1M
NIDDK NIH HHS U54 DK107965NIGMS NIH HHS R01 GM042516NIGMS NIH HHS R01 GM058460NIGMS NIH HHS R01 GM098319
6 · The paper itself

Abstract

Achieving stable expression of a single transgene in mammalian cells remains challenging; even more challenging is obtaining simultaneous stable expression of multiple transgenes at reproducible, relative expression levels. Previously, we attained copy-number-dependent, chromosome-position-independent expression of reporter minigenes by embedding them within a BAC "scaffold" containing the mouse

Indexed as

Chromosomes, Artificial, BacterialAnimalsGenes, ReporterGenetic EngineeringGreen Fluorescent ProteinsMiceMicroscopy, FluorescenceMutS Homolog 3 ProteinNIH 3T3 CellsPromoter Regions, GeneticTetrahydrofolate DehydrogenaseTransfectionTransgenesenhanced green fluorescent proteinGreen Fluorescent ProteinsMsh3 protein, mouseMutS Homolog 3 ProteinTetrahydrofolate DehydrogenaseBAC recombineeringcopy-number-dependent expressiongene silencingmultireporterpromoter toolkittransgene expression

Identifiers

PMID32216371
PMCPMC7230027
OpenAlexW3013116871

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.