ReviewApplied microbiology and biotechnology2020
Methods for detection of viable foodborne pathogens: current state-of-art and future prospects.
Review in Applied microbiology and biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 95 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
95 citing papers in PubMed, 1 synthesis or guideline pooled it, 280 citations in OpenAlex.
- AI-Enabled Imaging for Pathogen Detection Under Stress Conditions: A Systematic Review.Comprehensive reviews in food science and food safety · 2026Pooled it
- Smart Chemical Sensors for Monitoring and Detection of Spoilage in Fermented and Non-Fermented Food Products.Sensors (Basel, Switzerland) · 2026Review
- Sustainable Nanotechnology Approaches for Rapid Food Contaminant Detection and Future Food Safety Systems.Nanomaterials (Basel, Switzerland) · 2026Review
- Metagenomic profiling of bacterial (16S) and fungal (ITS) communities on d'Anjou pears during long-term controlled-atmosphere storage.Microbiology spectrum · 2026Article
- A Droplet Digital PCR Method for Simultaneous Detection and Quantification ofFoods (Basel, Switzerland) · 2026Article
- Implementing portable, real-time 16S rRNA sequencing in the healthcare sector enhances antimicrobial stewardship.EBioMedicine · 2026Article
- Article
- Molecular identification ofOpen veterinary journal · 2026Article
- Enzyme-enhanced RNA isolation from biofilm-producing bacteria.Microbiology spectrum · 2026Article
- Peroxidase-Mimicking Nanozymes for Rapid Detection of Infectious Diseases.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Stress-Driven Tolerance and Persistence ofBiology · 2026Review
- Prospective Study of the Relative Abundance of Antimicrobial Resistance Genes inAntibiotics (Basel, Switzerland) · 2026Article
- Limitations and safety aspects related to the use of bacteriophages in food production.FEMS microbiology reviews · 2026Article
- Recent Developments onFoods (Basel, Switzerland) · 2025Review
- Advances of MXene in Detection and Sterilization of Foodborne Pathogens.Foods (Basel, Switzerland) · 2025Review
- Portable AI-driven electrochemical aptasensor for real-time Staphylococcus aureus detection in food and beverages.Mikrochimica acta · 2025Article
- Proximity Ligation Assay: From a Foundational Principle to a Versatile Platform for Molecular and Translational Research.Biomolecules · 2025Review
- Integration of Artificial Intelligence in Biosensors for Enhanced Detection of Foodborne Pathogens.Biosensors · 2025Review
- Advancements in Microbial Applications for Sustainable Food Production.Foods (Basel, Switzerland) · 2025Review
- Bacteriophages combined with hybridized nanoflower-based electrochemical biosensor for the specific and rapid detection of live Acinetobacter baumannii in real samples.Mikrochimica acta · 2025Article
35 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to rapidly detect viable pathogens in food is important for public health and food safety reasons. Culture-based detection methods, the traditional means of demonstrating microbial viability, tend to be laborious, time consuming and slow to provide results. Several culture-independent methods to detect viable pathogens have been reported in recent years, including both nucleic acid-based (PCR combined with use of cell viability dyes or reverse-transcriptase PCR to detect messenger RNA) and phage-based (plaque assay or phage amplification and lysis plus PCR/qPCR, immunoassay or enzymatic assay to detect host DNA, progeny phages or intracellular components) methods. Some of these newer methods, particularly phage-based methods, show promise in terms of speed, sensitivity of detection and cost compared with culture for food testing. This review provides an overview of these new approaches and their food testing applications, and discusses their current limitations and future prospects in relation to detection of viable pathogens in food. KEY POINTS: • Cultural methods may be 'gold standard' for assessing viability of pathogens, but they are too slow. • Nucleic acid-based methods offer speed of detection but not consistently proof of cell viability. • Phage-based methods appear to offer best alternative to culture for detecting viable pathogens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.