Evidence map›Paper›PMID 32212719›Full record

ArticleAnalytical chemistry2020

Localization of Double Bonds in Bacterial Glycerophospholipids Using 193 nm Ultraviolet Photodissociation in the Negative Mode.

Dustin R Klein, Molly S Blevins, Luis A Macias, Martin V Douglass, M Stephen Trent, Jennifer S Brodbelt

Open access · greenAbstract read
In one paragraph

Article in Analytical chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 25 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Dustin R KleinDepartment of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States.
Molly S BlevinsDepartment of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States.
Luis A MaciasDepartment of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States.
Martin V DouglassDepartment of Infectious Diseases, The University of Georgia, College of Veterinary Medicine, Athens, Georgia 30602, United States.
M Stephen TrentDepartment of Infectious Diseases, The University of Georgia, College of Veterinary Medicine, Athens, Georgia 30602, United States.ORCID 0000-0001-6134-1800
Jennifer S BrodbeltDepartment of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States.ORCID 0000-0003-3207-0217
The University of Texas at Austin · USGeorgia College & State University · US

Funding

The Cell Envelope of the Multi-Drug Resistant Pathogen Acinetobacter baumanniiR01AI150098 · NIAID · UNIVERSITY OF GEORGIA · PI Michael Stephen Trent · 2020 to 2026
$3.9M
Development of a novel vaccine platform: Surface Antigen/Adjuvant Vaccine Engineering (SAAVE)R01AI129940 · NIAID · UNIVERSITY OF GEORGIA · PI TRENT, MICHAEL STEPHEN · 2017 to 2021
$3.3M
UVPD-MS Characterization of LipopolysaccharidesR01GM103655 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI BRODBELT, JENNIFER S. · 2012 to 2019
$2.3M
Molecular mechanisms required for the maintenance of the gram-negative outer membraneR01AI138576 · NIAID · UNIVERSITY OF GEORGIA · PI TRENT, MICHAEL STEPHEN · 2018 to 2022
$2.0M
Glycoscience Training ProgramT32GM107004 · NIGMS · UNIVERSITY OF GEORGIA · PI AMSTER, I JONATHAN, HALTIWANGER, ROBERT S. · 2014 to 2021
$1.3M
NIAID NIH HHS R01 AI129940NIAID NIH HHS R01 AI138576NIAID NIH HHS R01 AI150098NIGMS NIH HHS R01 GM103655NIGMS NIH HHS T32 GM107004
6 · The paper itself

Abstract

The need for detailed structural characterization of glycerophospholipids (GPLs) for many types of biologically motivated applications has led to the development of novel mass spectrometry-based methodologies that utilize alternative ion activation methods. Ultraviolet photodissociation (UVPD) has shown great utility for localizing sites of unsaturation within acyl chains and to date has predominantly been used for positive mode analysis of GPLs. In the present work, UVPD is used to localize sites of unsaturation in GPL anions. Similar to UVPD mass spectra of GPL cations, UVPD of deprotonated or formate-adducted GPLs yields diagnostic fragment ions spaced 24 Da apart. This method was integrated into a liquid chromatography workflow and used to evaluate profiles of sites of unsaturation of lipids in

Indexed as

Ultraviolet RaysAcinetobacter baumanniiBacterial ProteinsChromatography, LiquidEscherichia coliGlycerophospholipidsMass SpectrometryMolecular StructureBacterial ProteinsGlycerophospholipids

Identifiers

PMID32212719
PMCPMC7385702
OpenAlexW3013072031

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.