ArticleFrontiers in cellular and infection microbiology2020
Development of ELISA and Lateral Flow Immunoassays for Ochratoxins (OTA and OTB) Detection Based on Monoclonal Antibody.
Article in Frontiers in cellular and infection microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 55 citations in OpenAlex.
- Selective detection of Ochratoxin B in food by fluorescent sensor based on 2-mercapto-5-benzimidazole carboxylic acid-capped CdSe quantum dots.Food chemistry: X · 2026Article
- Tri-mode fluorescence/colorimetric/strip aptasensor with CRISPR/Cas12a-driven cascade strategy for mycotoxin.Current research in food science · 2026Article
- Toward smart and in-situ mycotoxin detection in food via vibrational spectroscopy and machine learning.Food chemistry: X · 2025Review
- Aspergillus Mycotoxins: The Major Food Contaminants.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Methodology and application of multiplex PCR-dipstick DNA chromatography for the detection of eight respiratory bacterial pathogens.Frontiers in cellular and infection microbiology · 2025Article
- Tetrodotoxin: The State-of-the-Art Progress in Characterization, Detection, Biosynthesis, and Transport Enrichment.Marine drugs · 2024Review
- Recent Advances in Surface Plasmon Resonance (SPR) Technology for Detecting Ovarian Cancer Biomarkers.Cancers · 2023Review
- An Extremely Highly Sensitive ELISA in pg mLMolecules (Basel, Switzerland) · 2023Article
- Review
- Smartphone-Based Quantitative Detection of Ochratoxin A in Wheat via a Lateral Flow Assay.Foods (Basel, Switzerland) · 2023Article
- Isolation and characterization of human anti-CD20 single-chain variable fragment (scFv) from a Naive human scFv library.Medical oncology (Northwood, London, England) · 2022Article
- Preparation of Monoclonal Antibody against Deoxynivalenol and Development of Immunoassays.Toxins · 2022Article
- Development of Immunochromatographic Strip for Detection of αB-VxXXIVA-Conotoxin Based on 5E4 Monoclonal Antibody.Toxins · 2022Article
- Detection of αB-Conotoxin VxXXIVA (αB-CTX) by ic-ELISA Based on an Epitope-Specific Monoclonal Antibody.Toxins · 2022Article
- Comparative Assessment of Different Gold Nanoflowers as Labels for Lateral Flow Immunosensors.Sensors (Basel, Switzerland) · 2021Article
- Scanning Electrochemical Impedance Microscopy in Redox-Competition Mode for the Investigation of Antibodies Labelled with Horseradish Peroxidase.Materials (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ochratoxins were important secondary metabolites secreted by fungi, and OTA and OTB are mainly significant mycotoxin, having toxic effects on humans and animals. Therefore, it is important to establish a rapid, sensitive, and precise method for ochratoxins detection and quantification in real samples. In this study, a stable monoclonal antibody (mAb) that recognizing both OTA and OTB toxins was employed for the establishment of indirect competitive ELISA (ic-ELISA), colloidal gold nanoparticles (CGNs), and nanoflowers gold strips (AuNFs) for detection of ochratoxins in real samples. A 6E5 hybridoma cell line stable secreting mAb against both OTA and OTB toxins was obtained by fusion of splenocytes with myeloma SP2/0 cells. The 6E5 mAb had a high affinity (3.7 × 10
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