ArticleThe Journal of biological chemistry2020
The small EF-hand protein CALML4 functions as a critical myosin light chain within the intermicrovillar adhesion complex.
Article in The Journal of biological chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 39 citations in OpenAlex.
- Metabolic and muscular adaptations to dietary succinate in Tan sheep: Insights from transcriptomics.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2026Article
- Tonotopic specialization of MYO7A isoforms in auditory hair cells.Nature communications · 2026Article
- Brush border intermicrovillar adhesion limits bacteria attachment to the small intestine brush border.bioRxiv : the preprint server for biology · 2025Article
- Comparative transcriptomic and machine learning analysis identifies key genes and immune dysregulation in goats exposed to peste des petits ruminants virus.Virus genes · 2025Article
- Injured Proximal Tubular Epithelial Cells Lose Hepatocyte Nuclear Factor 4α Expression Crucial for Brush Border Formation and Transport.The American journal of pathology · 2025Article
- The filopodial myosin DdMyo7 is a slow, calcium-regulated motor.The Journal of biological chemistry · 2025Article
- Select autosomal dominant DFNA11 deafness variants activate Myo7A targeting in epithelial cells.Journal of cell science · 2025Article
- Expression, Purification, and In Vitro Analysis of Myosin.Methods in molecular biology (Clifton, N.J.) · 2025Article
- The poly-proline motifs found in the cytoplasmic tail of the brush border cadherin CDHR5 play a role in its ability to elongate microvilli.microPublication biology · 2025Article
- Article
- Loss of intermicrovillar adhesion factor CDHR2 impairs basolateral junctional complexes in transporting epithelia.Molecular biology of the cell · 2024Article
- MultiBac System-based Purification and Biophysical Characterization of Human Myosin-7a.Journal of visualized experiments : JoVE · 2024Article
- Minocycline Inhibits Tick-Borne Encephalitis Virus and Protects Infected Cells via Multiple Pathways.Viruses · 2024Article
- Inhibition of Prolactin Affects Epididymal Morphology by Decreasing the Secretion of Estradiol in Cashmere Bucks.Animals : an open access journal from MDPI · 2024Article
- Epigenetic Modifications of White Blood Cell DNA Caused by Transient Fetal Infection with Bovine Viral Diarrhea Virus.Viruses · 2024Article
- The microvillar protocadherin CDHR5 associates with EBP50 to promote brush border assembly.Molecular biology of the cell · 2024Article
- Adhesion-based capture stabilizes nascent microvilli at epithelial cell junctions.Developmental cell · 2023Article
- Defects in microvillus crosslinking sensitize to colitis and inflammatory bowel disease.EMBO reports · 2023Article
- Heart rate monitoring to detect acute pain in non-verbal patients: a study protocol for a randomized controlled clinical trial.BMC psychiatry · 2023Article
- Biomimetic Remodeling of Microglial Riboflavin Metabolism Ameliorates Cognitive Impairment by Modulating Neuroinflammation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
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9 authors at 2 institutions in 1 country.
Funding
Abstract
Specialized transporting and sensory epithelial cells employ homologous protocadherin-based adhesion complexes to remodel their apical membrane protrusions into organized functional arrays. Within the intestine, the nutrient-transporting enterocytes utilize the intermicrovillar adhesion complex (IMAC) to assemble their apical microvilli into an ordered brush border. The IMAC bears remarkable homology to the Usher complex, whose disruption results in the sensory disorder type 1 Usher syndrome (USH1). However, the entire complement of proteins that comprise both the IMAC and Usher complex are not yet fully elucidated. Using a protein isolation strategy to recover the IMAC, we have identified the small EF-hand protein calmodulin-like protein 4 (CALML4) as an IMAC component. Consistent with this finding, we show that CALML4 exhibits marked enrichment at the distal tips of enterocyte microvilli, the site of IMAC function, and is a direct binding partner of the IMAC component myosin-7b. Moreover, distal tip enrichment of CALML4 is strictly dependent upon its association with myosin-7b, with CALML4 acting as a light chain for this myosin. We further show that genetic disruption of CALML4 within enterocytes results in brush border assembly defects that mirror the loss of other IMAC components and that CALML4 can also associate with the Usher complex component myosin-7a. Our study further defines the molecular composition and protein-protein interaction network of the IMAC and Usher complex and may also shed light on the etiology of the sensory disorder USH1H.
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