Evidence map›Paper›PMID 32209230›Full record

ArticleeLife2020

Building customizable auto-luminescent luciferase-based reporters in plants.

Arjun Khakhar, Colby G Starker, James C Chamness, Nayoung Lee, Sydney Stokke, Cecily Wang, Ryan Swanson, Furva Rizvi, Takato Imaizumi, Daniel F Voytas

Erratum issuedOpen access · goldAbstract read
In one paragraph

Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 48 papers.

0numbers the graph read from it
0cells of the map it votes in
48citing papers in PubMed
3.4field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

48 citing papers in PubMed, 82 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Sentinel plants enable quantitative monitoring of bioavailable nitrate in soils and microbial environments.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  5. Bioluminescence in the Edible MushroomJournal of fungi (Basel, Switzerland) · 2026
    Article
  6. Article
  7. Article
  8. Article
  9. Review
  10. Review
  11. Review
  12. Review
  13. Review
  14. Strengths and Limitations of Salicylic Acid Reporters.International journal of molecular sciences · 2025
    Review
  15. Article
  16. Synthesis of large single-transcript pathways from oligonucleotide pools: Design of STARBURST, an autobioluminescent reporter.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  17. Review
  18. Article
  19. Article
  20. Imaging Plant Lipids with Fluorescent Reporters.Plants (Basel, Switzerland) · 2024
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Arjun KhakharDepartment Genetics, Cell Biology, & Development, University of Minnesota, Minneapolis, United States.ORCID 0000-0002-4676-6533
Colby G StarkerDepartment Genetics, Cell Biology, & Development, University of Minnesota, Minneapolis, United States.ORCID 0000-0002-6774-7227
James C ChamnessDepartment Genetics, Cell Biology, & Development, University of Minnesota, Minneapolis, United States.
Nayoung LeeDepartment of Biology, University of Washington, Seattle, United States.
Sydney StokkeDepartment Genetics, Cell Biology, & Development, University of Minnesota, Minneapolis, United States.
Cecily WangDepartment Genetics, Cell Biology, & Development, University of Minnesota, Minneapolis, United States.
Ryan SwansonDepartment Genetics, Cell Biology, & Development, University of Minnesota, Minneapolis, United States.
Furva RizviDepartment Genetics, Cell Biology, & Development, University of Minnesota, Minneapolis, United States.
Takato ImaizumiDepartment of Biology, University of Washington, Seattle, United States.
Daniel F VoytasDepartment Genetics, Cell Biology, & Development, University of Minnesota, Minneapolis, United States.ORCID 0000-0002-4944-1224
University of Minnesota · USUniversity of Washington · US

Funding

Molecular Mechanisms of Seasonal Time MeasurementR01GM079712 · NIGMS · UNIVERSITY OF WASHINGTON · PI IMAIZUMI, TAKATO · 2007 to 2022
$3.5M
NIGMS NIH HHS R01 GM079712Rural Development Administration PJ013386
6 · The paper itself

Abstract

Bioluminescence is a powerful biological signal that scientists have repurposed as a reporter for gene expression in plants and animals. However, there are downsides associated with the need to provide a substrate to these reporters, including its high cost and non-uniform tissue penetration. In this work we reconstitute a fungal bioluminescence pathway (FBP)

Indexed as

LuminescenceLuminescent MeasurementsAnimalsFungiGene ExpressionLuciferasesPlantsLuciferasesA. thalianabioluminescenceexpression reporterhormone reportersluciferaseNicotiana benthamianaplant biologyplant synthetic biology

Identifiers

PMID32209230
PMCPMC7164954
OpenAlexW3013470802

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.