Evidence map›Paper›PMID 32208890›Full record

ArticleCell cycle (Georgetown, Tex.)2020

Knockdown of microRNA-584 promotes dental pulp stem cells proliferation by targeting TAZ.

Songbo Tian, Yanping Liu, Fusheng Dong, Yongqing Dou, Wenjing Li, Jie Wang

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell cycle (Georgetown, Tex.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed, 1 pooled it
1.4field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Review
  5. Review
  6. Review
  7. Review
  8. Review
  9. Review
  10. Review
  11. Review
  12. Review
  13. Review
  14. Review
  15. Review
  16. Article
  17. Article
  18. Review
  19. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Songbo TianDepartment of Oral Pathology, College of Stomatology, Hebei Medical University, Shijiazhuang, China.
Yanping LiuPhysical Examination Center, The Second Hospital of Hebei Medical University, Shijiazhuang, China.
Fusheng DongDepartment of Oral and Maxillofacial Surgery, College of Stomatology, Hebei Medical University, Shijiazhuang, China.
Yongqing DouCollege of Integrated Chinese and Western Medicine, Hebei University of Chinese Medicine, Shijiazhuang, China.
Wenjing LiDepartment of Oral Medicine, The Second Hospital of Hebei Medical University, Shijiazhuang, China.
Jie WangDepartment of Oral Pathology, College of Stomatology, Hebei Medical University, Shijiazhuang, China.
Hebei Medical University · CNSecond Hospital of Hebei Medical University · CNHebei University of Chinese Medicine · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Proliferation of dental pulp stem cells (DPSCs) is crucial in tooth development and damage repairing, also includes its therapy application for tissue engineering. MicroRNAs (miRNAs) are key players in biological processes of DPSCs, and transcriptional co-activator with PDZ-binding motif (TAZ) also plays important roles in cell proliferation and differentiation, however, the roles of miR-584 and TAZ in DPSCs are not known. We found up-regulated miR-584 expression and down-regulated TAZ expression levels in aging dental pulp tissue compare to those in young dental pulp tissue. In proliferating DPSCs we demonstrated the decreased miR-584 expression and increased TAZ expression. miR-584 mimics suppressed DPSCs proliferation and migration, and significantly reduced TAZ production, whereas miR-584 inhibition exerted the converse effects. Knocking down of the TAZ in DPSCs had a similar effect as overexpression of miR-584. Furthermore, luciferase reporter assay demonstrated that miR-584 could directly bind to the TAZ mRNA 3'UTR to repress its translation. Overexpression of TAZ can partly rescue miR-584 mimic-mediated the inhibition of proliferation. Additionally, miR-584 inhibited cell proliferation and downregulated expression of cell cycle proteins by AKT signaling pathway. Together, we identified that miR-584 may be a key regulator in the proliferation of DPSCs by regulating TAZ expression via AKT signaling pathway. It would be a promising biomarker and therapeutic target for pulp disease.

Indexed as

Gene Knockdown TechniquesAdolescentAgedAge FactorsBiomarkersCell ProliferationCells, CulturedChildDental PulpDental Pulp DiseasesHealthy VolunteersHumansIntracellular Signaling Peptides and ProteinsMaleMicroRNAsMiddle AgedBiomarkersIntracellular Signaling Peptides and ProteinsMicroRNAsMIRN584 microRNA, humanProto-Oncogene Proteins c-aktTranscriptional Coactivator with PDZ-Binding Motif ProteinsWWTR1 protein, humancell cycleDPSCsmiRNA-584proliferationTAZ

Identifiers

PMID32208890
PMCPMC7217370
OpenAlexW3013386263

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.