Evidence map›Paper›PMID 32207815›Full record

ArticleThe Biochemical journal2020

A bacteriophage mimic of the bacterial nucleoid-associated protein Fis.

Soumyananda Chakraborti, Dhanasekaran Balakrishnan, Alexander J Trotter, William H Gittens, Ally W H Yang, Arttu Jolma, Joy R Paterson, Sylwia Świątek, Jacek Plewka, Fiona A Curtis and 7 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Biochemical journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Genomic characterization of fourFood science and biotechnology · 2025
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 4 institutions in 3 countries.

Soumyananda ChakrabortiMalopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.
Dhanasekaran BalakrishnanMalopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.
Alexander J TrotterDepartment of Biosciences, Durham University, Stockton Road, Durham DH1 3LE, U.K.
William H GittensDepartment of Biosciences, Durham University, Stockton Road, Durham DH1 3LE, U.K.
Ally W H YangDonnelly Centre, University of Toronto, 160 College Street, Toronto, ON M5S 3E1, Canada.
Arttu JolmaDonnelly Centre, University of Toronto, 160 College Street, Toronto, ON M5S 3E1, Canada.
Joy R PatersonDepartment of Biosciences, Durham University, Stockton Road, Durham DH1 3LE, U.K.
Sylwia ŚwiątekMalopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.
Jacek PlewkaMalopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.
Fiona A CurtisDepartment of Biosciences, Durham University, Stockton Road, Durham DH1 3LE, U.K.
Laura Y BowersDepartment of Biosciences, Durham University, Stockton Road, Durham DH1 3LE, U.K.
Lars-Olof PålssonDepartment of Chemistry, Durham University, Stockton Road, Durham DH1 3LE, U.K.
Timothy R HughesDonnelly Centre, University of Toronto, 160 College Street, Toronto, ON M5S 3E1, Canada.
Michał TaubeDepartment of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University, Uniwersytetu Poznańskiego 2, PL 61-614 Poznań, Poland.
Maciej KozakDepartment of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University, Uniwersytetu Poznańskiego 2, PL 61-614 Poznań, Poland.
Jonathan G HeddleMalopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.
Gary J SharplesDepartment of Biosciences, Durham University, Stockton Road, Durham DH1 3LE, U.K.
Durham University · GBJagiellonian University · PLUniversity of Toronto · CAAdam Mickiewicz University in Poznań · PL

Funding

Biotechnology and Biological Sciences Research Council BB/C514523/1Biotechnology and Biological Sciences Research Council BB/F020503/1Biotechnology and Biological Sciences Research Council BB/FO20503/1CIHR FDN-138403
6 · The paper itself

Abstract

We report the identification and characterization of a bacteriophage λ-encoded protein, NinH. Sequence homology suggests similarity between NinH and Fis, a bacterial nucleoid-associated protein (NAP) involved in numerous DNA topology manipulations, including chromosome condensation, transcriptional regulation and phage site-specific recombination. We find that NinH functions as a homodimer and is able to bind and bend double-stranded DNA in vitro. Furthermore, NinH shows a preference for a 15 bp signature sequence related to the degenerate consensus favored by Fis. Structural studies reinforced the proposed similarity to Fis and supported the identification of residues involved in DNA binding which were demonstrated experimentally. Overexpression of NinH proved toxic and this correlated with its capacity to associate with DNA. NinH is the first example of a phage-encoded Fis-like NAP that likely influences phage excision-integration reactions or bacterial gene expression.

Indexed as

Bacterial ProteinsBacteriophage lambdaBase SequenceBinding SitesComputer SimulationDNADNA-Binding ProteinsDNA, ViralEscherichia coliEscherichia coli ProteinsFactor For Inversion Stimulation ProteinGene ExpressionMutant ProteinsProtein Conformation, alpha-HelicalProtein Conformation, beta-StrandProtein MultimerizationBacterial ProteinsDNADNA-Binding ProteinsDNA, ViralEscherichia coli ProteinsFactor For Inversion Stimulation ProteinFis protein, E coliH-NS protein, bacteriaMutant ProteinsViral Proteinsbacteriophage λDNA bendingDNA bindingFisNinHnucleoid-associated proteins

Identifiers

PMID32207815
PMCPMC7166090
OpenAlexW3012900057

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.