Evidence map›Paper›PMID 32206968›Full record

ArticleGeroScience2020

A novel tissue culture model for evaluating the effect of aging on stem cell fate in adult microvascular networks.

Mohammad S Azimi, Jessica M Motherwell, Maria Dutreil, Ryan L Fishel, Matthew Nice, Nicholas A Hodges, Bruce A Bunnell, Adam Katz, Walter L Murfee

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in GeroScience, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Article
  3. State of the field: cellular and exosomal therapeutic approaches in vascular regeneration.American journal of physiology. Heart and circulatory physiology · 2022
    Review
  4. Review
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Mohammad S AzimiDepartment of Biomedical Engineering, Tulane University, New Orleans, LA, 70118, USA.
Jessica M MotherwellDepartment of Biomedical Engineering, Tulane University, New Orleans, LA, 70118, USA.
Maria DutreilTulane Center for Stem Cell Research & Regenerative Medicine, Tulane University School of Medicine, New Orleans, LA, 70112, USA.
Ryan L FishelDepartment of Biomedical Engineering, Tulane University, New Orleans, LA, 70118, USA.
Matthew NiceDepartment of Biomedical Engineering, Tulane University, New Orleans, LA, 70118, USA.
Nicholas A HodgesDepartment of Biomedical Engineering, Tulane University, New Orleans, LA, 70118, USA.
Bruce A BunnellTulane Center for Stem Cell Research & Regenerative Medicine, Tulane University School of Medicine, New Orleans, LA, 70112, USA.
Adam KatzDepart of Surgery, University of Florida School of Medicine, Gainesville, FL, 32611, USA.
Walter L MurfeeJ. Crayton Pruitt Department of Biomedical Engineering, University of Florida, Gainesville, FL, 32611, USA. wmurfee@bme.ufl.edu.
Tulane University · USUniversity of Florida · US

Funding

Angiogenesis Model for Aging ResearchR01AG049821 · NIA · UNIVERSITY OF FLORIDA · PI MURFEE, WALTER L · 2016 to 2019
$1.0M
NIA NIH HHS R01 AG049821NIA NIH HHS R01AG049821
6 · The paper itself

Abstract

In vitro models of angiogenesis are valuable tools for understanding the underlying mechanisms of pathological conditions and for the preclinical evaluation of therapies. Our laboratory developed the rat mesentery culture model as a new tool for investigating mechanistic cell-cell interactions at specific locations across intact blood and lymphatic microvascular networks ex vivo. The objective of this study was to report a method for evaluating the effect of aging on human stem cell differentiation into pericytes during angiogenesis in cultured microvascular networks. DiI labeled exogenous stem cells were seeded onto harvested adult Wistar rat mesenteric tissues and cultured in alpha-MEM + 1% serum for up to 5 days according to four experimental groups: (1) adult human adipose-derived stem cells (hASCs), (2) aged hASCs, (3) adult human bone marrow-derived stem cells (hBMSCs), and (4) aged hBMSCs. Angiogenesis per experimental group was supported by observation of increased vessel density and capillary sprouting. For each tissue per experimental group, a subset of cells was observed in typical pericyte location wrapped along blood vessels. Stem cell differentiation into pericytes was supported by the adoption of elongated pericyte morphology along endothelial cells and positive NG2 labeling. The percentage of cells in pericyte locations was not significantly different across the experimental groups, suggesting that aged mesenchymal stem cells are able to retain their differentiation capacity. Our results showcase an application of the rat mesentery culture model for aging research and the evaluation of stem cell fate within intact microvascular networks.

Indexed as

AgingEndothelial CellsNeovascularization, PhysiologicStem CellsAnimalsCell DifferentiationMicrovesselsRatsRats, WistarTissue Culture TechniquesAgingAngiogenesisBiomimetic modelMicrocirculationPericyteStem cell

Identifiers

PMID32206968
PMCPMC7205973
OpenAlexW3012733529

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.