ArticleScience advances2020
Recapitulating complex biological signaling environments using a multiplexed, DNA-patterning approach.
Article in Science advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- Low-Cost Open Platform Digital Light Printer (OP-DLP) for 96-Well Format Hydrogel Printing and Localized Light-Activation.ACS biomaterials science & engineering · 2026Article
- DNA-programmed cell assembly: from cells, tissues to organoids.Frontiers in bioengineering and biotechnology · 2025Review
- Controlling Cell Interactions with DNA Directed Assembly.Advanced healthcare materials · 2024Review
- Advances in high throughput cell culture technologies for therapeutic screening and biological discovery applications.Bioengineering & translational medicine · 2024Review
- Nanopatterned Monolayers of Bioinspired, Sequence-Defined Polypeptoid Brushes for Semiconductor/Bio Interfaces.ACS nano · 2024Article
- High-Throughput Assembly of Compositionally Controlled 3D Cell Communities for Developmental Engineering.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Light-Controlled Cell-Cell Assembly Using Photocaged Oligonucleotides.ACS materials Au · 2023Article
- Independent control over cell patterning and adhesion on hydrogel substrates for tissue interface mechanobiology.iScience · 2023Article
- Programming the Self-Organization of Endothelial Cells into Perfusable Microvasculature.Tissue engineering. Part A · 2023Article
- DynamicNano letters · 2022Article
- A reductionist approach to determine the effect of cell-cell contact on human epidermal stem cell differentiation.Acta biomaterialia · 2022Article
- Self-assembly of tessellated tissue sheets by expansion and collision.Nature communications · 2022Article
- Detecting Intact Virus Using Exogenous Oligonucleotide Labels.Analytical chemistry · 2022Article
- DNA-Directed Patterning for Versatile Validation and Characterization of a Lipid-Based Nanoparticle Model of SARS-CoV-2.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Multiplexed DNA-Directed Patterning of Antibodies for Applications in Cell Subpopulation Analysis.ACS applied materials & interfaces · 2021Article
- Expanding the boundaries of synthetic development.Developmental biology · 2021Review
- Simple, Affordable, and Modular Patterning of Cells using DNA.Journal of visualized experiments : JoVE · 2021Article
- Bioprinting for the Biologist.Cell · 2021Review
- Guiding Cell Network Assembly using Shape-Morphing Hydrogels.Advanced materials (Deerfield Beach, Fla.) · 2020Article
- Harnessing the Power of Eph/ephrin Biosemiotics for Theranostic Applications.Pharmaceuticals (Basel, Switzerland) · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
Elucidating how the spatial organization of extrinsic signals modulates cell behavior and drives biological processes remains largely unexplored because of challenges in controlling spatial patterning of multiple microenvironmental cues in vitro. Here, we describe a high-throughput method that directs simultaneous assembly of multiple cell types and solid-phase ligands across length scales within minutes. Our method involves lithographically defining hierarchical patterns of unique DNA oligonucleotides to which complementary strands, attached to cells and ligands-of-interest, hybridize. Highlighting our method's power, we investigated how the spatial presentation of self-renewal ligand fibroblast growth factor-2 (FGF-2) and differentiation signal ephrin-B2 instruct single adult neural stem cell (NSC) fate. We found that NSCs have a strong spatial bias toward FGF-2 and identified an unexpected subpopulation exhibiting high neuronal differentiation despite spatially occupying patterned FGF-2 regions. Overall, our broadly applicable, DNA-directed approach enables mechanistic insight into how tissues encode regulatory information through the spatial presentation of heterogeneous signals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.