Evidence map›Paper›PMID 32206400›Full record

ArticleBiomedical optics express2020

Raster adaptive optics for video rate aberration correction and large FOV multiphoton imaging.

Yongxiao Li, Yean J Lim, Qiongkai Xu, Lynette Beattie, Elizabeth E Gardiner, Katharina Gaus, William R Heath, Woei Ming Lee

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In one paragraph

Article in Biomedical optics express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Fast wavefront shaping for two-photon brain imaging with multipatch correction.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  2. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yongxiao LiResearch School of Electrical, Energy and Materials Engineering, College of Engineering and Computer Science, The Australian National University, 31 North Road, Canberra, ACT, 2601, Australia.
Yean J LimResearch School of Electrical, Energy and Materials Engineering, College of Engineering and Computer Science, The Australian National University, 31 North Road, Canberra, ACT, 2601, Australia.
Qiongkai XuResearch School of Computer Science, College of Engineering and Computer Science, The Australian National University, 31 North Road, Canberra, ACT, 2601, Australia.
Lynette BeattieDepartment of Microbiology and Immunology, The University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, 3000, Australia.
Elizabeth E GardinerACRF Department of Cancer Biology and Therapeutics, The John Curtin School of Medical Research, The Australian National University, 131 Garran Road, Canberra, ACT, 2601, Australia.
Katharina GausEMBL Australia Node in Single Molecule Science and ARC Centre of Excellence in Advanced Molecular Imaging, The University of New South Wales, NSW, 2052, Australia.
William R HeathDepartment of Microbiology and Immunology, The University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, 3000, Australia.
Woei Ming LeeResearch School of Electrical, Energy and Materials Engineering, College of Engineering and Computer Science, The Australian National University, 31 North Road, Canberra, ACT, 2601, Australia.ORCID https://orcid.org/0000-0002-3912-6095

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Removal of complex aberrations at millisecond time scales over millimeters in distance in multiphoton laser scanning microscopy limits the total spatiotemporal imaging throughput for deep tissue imaging. Using a single low resolution deformable mirror and time multiplexing (TM) adaptive optics, we demonstrate video rate aberration correction (5 ms update rate for a single wavefront mask) for a complex heterogeneous distribution of refractive index differences through a depth of up to 1.1 mm and an extended imaging FOV of up to 0.8 mm, with up to 167% recovery of fluorescence intensity 335 µm from the center of the FOV. The proposed approach, termed raster adaptive optics (RAO), integrates image-based aberration retrieval and video rate removal of arbitrarily defined regions of dominant, spatially varied wavefronts. The extended FOV was achieved by demonstrating rapid recovery of up to 50 distinct wavefront masks at 500 ms update rates that increased imaging throughput by 2.3-fold. Because RAO only requires a single deformable mirror with image-based aberration retrieval, it can be directly implemented on a standard laser scanning multiphoton microscope.

Identifiers

PMID32206400
PMCPMC7041464

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.