ArticleBiomedical optics express2020
Defocused imaging exploits supercritical-angle fluorescence emission for precise axial single molecule localization microscopy.
Article in Biomedical optics express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed.
- Refractive Index Mapping below the Diffraction Limit via Single Molecule Localization Microscopy.ACS nano · 2026Article
- Combinatorial sample- and back-focal-plane imaging. Pt. I: Instrument and acquisition parameters affecting BFP images and their analysis.Biophysical journal · 2025Article
- Enhanced supercritical angle localization microscopy through point spread function modeling.Biomedical optics express · 2025Article
- Joint estimation of point spread function and molecule positions in SMLM informed from multiple planes.Biomedical optics express · 2025Article
- Three-Dimensional Single Molecule Localization Microscopy Reveals the Topography of the Immunological Synapse at Isotropic Precision below 15 nm.Nano letters · 2021Article
- Light scattering in TIRF microscopy: A theoretical study of the limits to surface selectivity.Biophysical journal · 2021Article
- Direct supercritical angle localization microscopy for nanometer 3D superresolution.Nature communications · 2021Article
- Three-dimensional single molecule localization close to the coverslip: a comparison of methods exploiting supercritical angle fluorescence.Biomedical optics express · 2021Article
- Erratum: Defocused imaging exploits supercritical-angle fluorescence emission for precise axial single molecule localization microscopy: erratum.Biomedical optics express · 2020Article
- Addressing systematic errors in axial distance measurements in single-emitter localization microscopy.Optics express · 2020Article
- 25Biomedical optics express · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
Abstract
Single molecule localization microscopy (SMLM) is one of the key techniques that break the classical resolution limit in optical imaging. It is based on taking multiple recordings of a sample, each showing only a sparse arrangement of spatially well separated fluorescent molecules which can be localized at nanometer precision. While localizing along the lateral directions is usually straightforward, estimating axial positions at a comparable precision is known to be much harder, which is due to the relatively large depth of focus provided by the microscope optics. Whenever a molecule is sufficiently close to the coverslip, it becomes feasible to draw additional information from near field coupling effects: super-critical angle fluorescence (SAF) appears and can be exploited to boost the axial localization precision. Here we propose defocused imaging as a SMLM strategy that is capable of leveraging the information contained in SAF. We show that, regarding axial localization precision, our approach is superior to established SAF-based approaches. At the same time it is simple and can be conducted on any research-grade microscope where controlled defocusing on the order of a few hundred nanometers is possible.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.