Evidence map›Paper›PMID 32206116›Full record

ReviewTheranostics2020

Progress, opportunity, and perspective on exosome isolation - efforts for efficient exosome-based theranostics.

Dongbin Yang, Weihong Zhang, Huanyun Zhang, Fengqiu Zhang, Lanmei Chen, Lixia Ma, Leon M Larcher, Suxiang Chen, Nan Liu, Qingxia Zhao and 4 more

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Theranostics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 581 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
581citing papers in PubMed, 4 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

581 citing papers in PubMed, 4 syntheses or guidelines pooled it.

  1. Pooled it
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  13. Natural Product-Derived Vesicles in Nanotherapeutics.Journal of extracellular vesicles · 2026
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521 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Dongbin YangDepartment of Neurosurgery of Hebi People's Hospital; Hebi Neuroanatomical Laboratory, Hebi, 458030, China.
Weihong ZhangSchool of Nursing, Zhengzhou University, Zhengzhou, 450001, China.
Huanyun ZhangDepartment of Neurosurgery of Hebi People's Hospital; Hebi Neuroanatomical Laboratory, Hebi, 458030, China.
Fengqiu ZhangHenan Key Laboratory of Ion-beam Bioengineering, Zhengzhou University, Zhengzhou, China, 450000.
Lanmei ChenGuangdong Key Laboratory for Research and Development of Nature Drugs, School of Pharmacy, Guangdong Medical University, Zhanjiang 524023, China.
Lixia MaSchool of Statistics, Henan University of Economics and Law, Zhengzhou 450046, China.
Leon M LarcherCentre for Molecular Medicine and Innovative Therapeutics, Murdoch University, Perth 6150, Australia.
Suxiang ChenCentre for Molecular Medicine and Innovative Therapeutics, Murdoch University, Perth 6150, Australia.
Nan LiuGeneral Practice Centre, Nanhai Hospital, Southern Medical University, 528244, Foshan, China.
Qingxia ZhaoSchool of Medicine, Wake Forest University, Winston Salem, NC 27101, USA.
Phuong H L TranSchool of Medicine, and Centre for Molecular and Medical Research, Deakin University, 3216, Australia.
Changying ChenThe First Affiliated Hospital of Zheng Zhou University, Zhengzhou 450001, China.
Rakesh N VeeduCentre for Molecular Medicine and Innovative Therapeutics, Murdoch University, Perth 6150, Australia.
Tao WangSchool of Nursing, Zhengzhou University, Zhengzhou, 450001, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Exosomes are small extracellular vesicles with diameters of 30-150 nm. In both physiological and pathological conditions, nearly all types of cells can release exosomes, which play important roles in cell communication and epigenetic regulation by transporting crucial protein and genetic materials such as miRNA, mRNA, and DNA. Consequently, exosome-based disease diagnosis and therapeutic methods have been intensively investigated. However, as in any natural science field, the in-depth investigation of exosomes relies heavily on technological advances. Historically, the two main technical hindrances that have restricted the basic and applied researches of exosomes include, first, how to simplify the extraction and improve the yield of exosomes and, second, how to effectively distinguish exosomes from other extracellular vesicles, especially functional microvesicles. Over the past few decades, although a standardized exosome isolation method has still not become available, a number of techniques have been established through exploration of the biochemical and physicochemical features of exosomes. In this work, by comprehensively analyzing the progresses in exosome separation strategies, we provide a panoramic view of current exosome isolation techniques, providing perspectives toward the development of novel approaches for high-efficient exosome isolation from various types of biological matrices. In addition, from the perspective of exosome-based diagnosis and therapeutics, we emphasize the issue of quantitative exosome and microvesicle separation.

Indexed as

ExosomesPrecision MedicineBiomarkersCell Line, TumorChromatography, GelHumansImmunoprecipitationMicrofluidic Analytical TechniquesUltrafiltrationBiomarkersdiagnosisExosomeextracellular vesiclemicrofluidicmicrovesicleseparation

Identifiers

PMID32206116
PMCPMC7069071

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.